http://purl.org/np/RA3MmCHHYDKYpNg2eP3Tmjn2QR_wpZXHhDjJnnCsuLFY8#Head
http://purl.org/np/RA3MmCHHYDKYpNg2eP3Tmjn2QR_wpZXHhDjJnnCsuLFY8
http://www.nanopub.org/nschema#hasAssertion
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http://purl.org/np/RA3MmCHHYDKYpNg2eP3Tmjn2QR_wpZXHhDjJnnCsuLFY8
http://www.nanopub.org/nschema#hasProvenance
http://purl.org/np/RA3MmCHHYDKYpNg2eP3Tmjn2QR_wpZXHhDjJnnCsuLFY8#provenance
http://purl.org/np/RA3MmCHHYDKYpNg2eP3Tmjn2QR_wpZXHhDjJnnCsuLFY8
http://www.nanopub.org/nschema#hasPublicationInfo
http://purl.org/np/RA3MmCHHYDKYpNg2eP3Tmjn2QR_wpZXHhDjJnnCsuLFY8#pubInfo
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http://www.w3.org/1999/02/22-rdf-syntax-ns#type
http://www.nanopub.org/nschema#Nanopublication
http://purl.org/np/RA3MmCHHYDKYpNg2eP3Tmjn2QR_wpZXHhDjJnnCsuLFY8#assertion
http://purl.obolibrary.org/obo/CHEBI_17992
http://www.w3.org/2000/01/rdf-schema#label
100mM sucrose
http://purl.obolibrary.org/obo/CHEBI_29234
http://www.w3.org/2000/01/rdf-schema#label
1% Triton -X
http://purl.obolibrary.org/obo/CHEBI_8984
http://www.w3.org/2000/01/rdf-schema#label
1% SDS
http://purl.org/np/RA3MmCHHYDKYpNg2eP3Tmjn2QR_wpZXHhDjJnnCsuLFY8#_1
http://www.w3.org/2000/01/rdf-schema#label
mosquitoes
http://purl.org/np/RA3MmCHHYDKYpNg2eP3Tmjn2QR_wpZXHhDjJnnCsuLFY8#_2
http://www.w3.org/2000/01/rdf-schema#label
mosquitoes
http://purl.org/np/RA3MmCHHYDKYpNg2eP3Tmjn2QR_wpZXHhDjJnnCsuLFY8#_3
http://www.w3.org/2000/01/rdf-schema#label
micropistle
http://purl.org/np/RA3MmCHHYDKYpNg2eP3Tmjn2QR_wpZXHhDjJnnCsuLFY8#_4
http://www.w3.org/2000/01/rdf-schema#label
Rnase A (20mg/ml)
http://purl.org/np/RA3MmCHHYDKYpNg2eP3Tmjn2QR_wpZXHhDjJnnCsuLFY8#_5
http://www.w3.org/2000/01/rdf-schema#label
1X STE buffer
http://purl.org/np/RA3MmCHHYDKYpNg2eP3Tmjn2QR_wpZXHhDjJnnCsuLFY8#_6
http://www.w3.org/2000/01/rdf-schema#label
Proteinase K (20mg/ml)
http://purl.org/np/RA3MmCHHYDKYpNg2eP3Tmjn2QR_wpZXHhDjJnnCsuLFY8#step
http://purl.org/dc/elements/1.1/description
Grind mosquitoes in 1.5 ml eppendorf tube with micropistle in 50-100 µl 1X STE buffer ( 50mM Nacl,50mM Tris- HCL,100mM EDTA, Ph 8.0) along with 100mM sucrose. Add 1X STE buffer to a total volume of 300-500 µl for a single mosquito and 1 ml for mosquito pool like 4,6,8,10 numbers. Then add 1% SDS ,1% Triton -X , 10 µl/ ml Rnase A (20mg/ml), 20 µl/ ml Proteinase K (20mg/ml) and mix it.
http://purl.org/np/RA3MmCHHYDKYpNg2eP3Tmjn2QR_wpZXHhDjJnnCsuLFY8#step
http://purl.org/net/SMARTprotocol#hasExperimentalInput
http://purl.org/np/RA3MmCHHYDKYpNg2eP3Tmjn2QR_wpZXHhDjJnnCsuLFY8#_1
http://purl.org/np/RA3MmCHHYDKYpNg2eP3Tmjn2QR_wpZXHhDjJnnCsuLFY8#step
http://www.w3.org/1999/02/22-rdf-syntax-ns#type
http://purl.org/net/p-plan#Step
http://purl.org/np/RA3MmCHHYDKYpNg2eP3Tmjn2QR_wpZXHhDjJnnCsuLFY8#step
https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#bioSampleUsed
http://purl.org/np/RA3MmCHHYDKYpNg2eP3Tmjn2QR_wpZXHhDjJnnCsuLFY8#_2
http://purl.org/np/RA3MmCHHYDKYpNg2eP3Tmjn2QR_wpZXHhDjJnnCsuLFY8#step
https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#labEquipmentUsed
http://purl.org/np/RA3MmCHHYDKYpNg2eP3Tmjn2QR_wpZXHhDjJnnCsuLFY8#_3
http://purl.org/np/RA3MmCHHYDKYpNg2eP3Tmjn2QR_wpZXHhDjJnnCsuLFY8#step
https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#labEquipmentUsed
http://www.bioassayontology.org/bao#BAO_0010014
http://purl.org/np/RA3MmCHHYDKYpNg2eP3Tmjn2QR_wpZXHhDjJnnCsuLFY8#step
https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#reagentUsed
http://purl.obolibrary.org/obo/CHEBI_17992
http://purl.org/np/RA3MmCHHYDKYpNg2eP3Tmjn2QR_wpZXHhDjJnnCsuLFY8#step
https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#reagentUsed
http://purl.obolibrary.org/obo/CHEBI_29234
http://purl.org/np/RA3MmCHHYDKYpNg2eP3Tmjn2QR_wpZXHhDjJnnCsuLFY8#step
https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#reagentUsed
http://purl.obolibrary.org/obo/CHEBI_8984
http://purl.org/np/RA3MmCHHYDKYpNg2eP3Tmjn2QR_wpZXHhDjJnnCsuLFY8#step
https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#reagentUsed
http://purl.org/np/RA3MmCHHYDKYpNg2eP3Tmjn2QR_wpZXHhDjJnnCsuLFY8#_4
http://purl.org/np/RA3MmCHHYDKYpNg2eP3Tmjn2QR_wpZXHhDjJnnCsuLFY8#step
https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#reagentUsed
http://purl.org/np/RA3MmCHHYDKYpNg2eP3Tmjn2QR_wpZXHhDjJnnCsuLFY8#_5
http://purl.org/np/RA3MmCHHYDKYpNg2eP3Tmjn2QR_wpZXHhDjJnnCsuLFY8#step
https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#reagentUsed
http://purl.org/np/RA3MmCHHYDKYpNg2eP3Tmjn2QR_wpZXHhDjJnnCsuLFY8#_6
http://www.bioassayontology.org/bao#BAO_0010014
http://www.w3.org/2000/01/rdf-schema#label
1.5 ml eppendorf tube
http://purl.org/np/RA3MmCHHYDKYpNg2eP3Tmjn2QR_wpZXHhDjJnnCsuLFY8#provenance
http://purl.org/np/RA3MmCHHYDKYpNg2eP3Tmjn2QR_wpZXHhDjJnnCsuLFY8#assertion
http://purl.org/pav/createdWith
https://nanotate.bitsfetch.com
http://purl.org/np/RA3MmCHHYDKYpNg2eP3Tmjn2QR_wpZXHhDjJnnCsuLFY8#assertion
http://www.w3.org/ns/prov#generatedAtTime
2021-03-16T16:48:04.108013
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http://purl.org/np/RA3MmCHHYDKYpNg2eP3Tmjn2QR_wpZXHhDjJnnCsuLFY8#assertion
http://www.w3.org/ns/prov#wasDerivedFrom
https://hypothes.is/a/8Y0HtIJ9EeuxMKftfvVUrw
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http://www.w3.org/ns/prov#wasDerivedFrom
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http://purl.org/np/RA3MmCHHYDKYpNg2eP3Tmjn2QR_wpZXHhDjJnnCsuLFY8#assertion
http://www.w3.org/ns/prov#wasDerivedFrom
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http://www.w3.org/ns/prov#wasDerivedFrom
https://hypothes.is/a/JzPoLoJ-EeubeTMBRz-oHg
http://purl.org/np/RA3MmCHHYDKYpNg2eP3Tmjn2QR_wpZXHhDjJnnCsuLFY8#assertion
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http://purl.org/np/RA3MmCHHYDKYpNg2eP3Tmjn2QR_wpZXHhDjJnnCsuLFY8#assertion
http://www.w3.org/ns/prov#wasDerivedFrom
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http://www.w3.org/ns/prov#wasDerivedFrom
https://hypothes.is/a/dCvMjIJ-EeuYqLMMrOutfQ
http://purl.org/np/RA3MmCHHYDKYpNg2eP3Tmjn2QR_wpZXHhDjJnnCsuLFY8#assertion
http://www.w3.org/ns/prov#wasDerivedFrom
https://hypothes.is/a/i-hYaIJ-EeuaRdNsg6w5Hg
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RSA
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http://purl.org/nanopub/x/hasPublicKey
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http://purl.org/np/RA3MmCHHYDKYpNg2eP3Tmjn2QR_wpZXHhDjJnnCsuLFY8#sig
http://purl.org/nanopub/x/hasSignature
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http://purl.org/np/RA3MmCHHYDKYpNg2eP3Tmjn2QR_wpZXHhDjJnnCsuLFY8
http://purl.org/nanopub/x/introduces
http://purl.org/np/RA3MmCHHYDKYpNg2eP3Tmjn2QR_wpZXHhDjJnnCsuLFY8#step
http://purl.org/np/RA3MmCHHYDKYpNg2eP3Tmjn2QR_wpZXHhDjJnnCsuLFY8
http://www.w3.org/ns/prov#generatedAtTime
2021-03-16T16:48:04.108013
http://purl.org/np/RA3MmCHHYDKYpNg2eP3Tmjn2QR_wpZXHhDjJnnCsuLFY8
http://www.w3.org/ns/prov#wasAttributedTo
https://hypothes.is/users/iannotate