@prefix pplan: <http://purl.org/net/p-plan#> .
@prefix this: <http://purl.org/np/RA6Ifadb3DQXlfrN41pl5iozjHrj4LMNhLm6z74BM8c6Q> .
@prefix sub: <http://purl.org/np/RA6Ifadb3DQXlfrN41pl5iozjHrj4LMNhLm6z74BM8c6Q#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix sp: <http://purl.org/net/SMARTprotocol#> .
@prefix dc: <http://purl.org/dc/elements/1.1/> .

sub:Head {
  this: np:hasAssertion sub:assertion;
    np:hasProvenance sub:provenance;
    np:hasPublicationInfo sub:pubInfo;
    a np:Nanopublication .
}

sub:assertion {
  <http://purl.obolibrary.org/obo/CHEBI_16991> rdfs:label "DNA" .
  
  sub:_1 rdfs:label "pellet" .
  
  sub:_2 rdfs:label "nuclease free water" .
  
  sub:_3 rdfs:label "TE buffer" .
  
  sub:step dc:description "Dissolve the dry pellet in nuclease free water or TE buffer (Ph 8.0). Store DNA at 4 º celcius  or -20 º celcius .";
    sp:hasExperimentalOutput <http://purl.obolibrary.org/obo/CHEBI_16991>;
    a pplan:Step;
    bs:bioSampleUsed <http://purl.obolibrary.org/obo/CHEBI_16991>, sub:_1;
    bs:reagentUsed sub:_2, sub:_3 .
}

sub:provenance {
  sub:assertion pav:createdWith <https://nanotate.bitsfetch.com>;
    prov:generatedAtTime "2021-03-18T06:34:27.555638"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/zuluaga.lizzz> .
}

sub:pubInfo {
  sub:assertion prov:wasDerivedFrom authority:IMGwSnbiEeuyhtu9n7srbg, authority:NDLvkHbiEeuvGws9CpXYEg,
      authority:RxAaSHbiEeuf9Qs7D-7mbQ, authority:W5TnvnbiEeu9zJfZKE5SbA, authority:cQA86HbiEeuDoKPmfzScPg .
  
  sub:sig npx:hasAlgorithm "RSA";
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB";
    npx:hasSignature "J+pIqaYp6r29FD+Cf8IDRc3qDXMpfffAnIw818hyZ2i2fDV1gmfh0pC/4Rw6Vo31tQx5/+4wK8dWmMh8LXnwhGkkEuUzmWyO8NMVaDzhajvOUmJMpFMEXwoRNiBYPwBqar3atmSQQ50BOJ98IiGqUewiZZiYoogn+3na3q+Tdag=";
    npx:hasSignatureTarget this: .
  
  this: npx:introduces sub:step;
    prov:generatedAtTime "2021-03-18T06:34:27.555638"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/zuluaga.lizzz> .
}