@prefix obo: <http://purl.obolibrary.org/obo/> .
@prefix dce: <http://purl.org/dc/elements/1.1/> .
@prefix pplan: <http://purl.org/net/p-plan#> .
@prefix this: <http://purl.org/np/RAAb0oBQSjGFE8J9e7QQ64pIzSw6Zf-wghGZap0bk3Pyg> .
@prefix sub: <http://purl.org/np/RAAb0oBQSjGFE8J9e7QQ64pIzSw6Zf-wghGZap0bk3Pyg#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix sp: <http://purl.org/net/SMARTprotocol#> .

sub:Head {
  this: np:hasAssertion sub:assertion;
    np:hasProvenance sub:provenance;
    np:hasPublicationInfo sub:pubInfo;
    a np:Nanopublication .
}

sub:assertion {
  obo:CHEBI_16991 rdfs:label "DNA" .
  
  sp:SeventyFivePercentEthanol rdfs:label "75% ethanol." .
  
  sub:step dce:description "Wash DNA pellet by adding 10 ml of 75% ethanol. Centrifuge at 12,000 x g for 10 min, 4 °C.";
    sp:hasExperimentalOutput obo:CHEBI_16991;
    a pplan:Step;
    bs:bioSampleUsed obo:CHEBI_16991;
    bs:reagentUsed sp:SeventyFivePercentEthanol .
}

sub:provenance {
  sub:assertion pav:createdWith <https://nanotate.bitsfetch.com>;
    prov:generatedAtTime "2021-02-26T09:48:23.647434"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/zuluaga.lizzz> .
}

sub:pubInfo {
  sub:assertion prov:wasDerivedFrom authority:1W95jng-EeupiidDEq_igg, authority:rE6wTHg-EeubtM9pye88Nw,
      authority:wPdFBHg-EeuUE88CGquTDg .
  
  sub:sig npx:hasAlgorithm "RSA";
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB";
    npx:hasSignature "WKwJTnsFWqiS87bQEtLHaya3UG3Y8wo5U484mWbhl5BY6W1ulAu41X7eFaLqrm7I6zFgkBKKdNyJfR3tjOfc9w1Id91G1thzsh36v+2dT+FeZ3c/Wy29caQ0+b7S9MidH6VYcO6sqOdIuoFG9eF+6MkjwPd3fM2W1PE6Mr0Gzms=";
    npx:hasSignatureTarget this: .
  
  this: npx:introduces sub:step;
    prov:generatedAtTime "2021-02-26T09:48:23.647434"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/zuluaga.lizzz> .
}