@prefix dce: .
@prefix pplan: .
@prefix this: .
@prefix sub: .
@prefix bs: .
@prefix np: .
@prefix pav: .
@prefix authority: .
@prefix npx: .
@prefix xsd: .
@prefix rdfs: .
@prefix prov: .
@prefix sp: .
sub:Head {
this: np:hasAssertion sub:assertion;
np:hasProvenance sub:provenance;
np:hasPublicationInfo sub:pubInfo;
a np:Nanopublication .
}
sub:assertion {
sub:_1 rdfs:label "leaf tissues" .
sub:_2 rdfs:label "leaf tissues" .
sub:_3 rdfs:label " 50 mL Falcon tube" .
sub:_4 rdfs:label "extraction buffer " .
sub:step dce:description "Take one gram of frozen finely powdered leaf tissues in a new 50 mL Falcon tube and mixed with the pre-heated extraction buffer (10 ml) for one sample. Breifly, vertex for 30sec to ensure leaf material is fully mixed with buffer. Fine grind is a key to obtaining high DNA quantity with lesser artifacts of resin.";
sp:hasExperimentalInput sub:_1;
a pplan:Step;
bs:bioSampleUsed sub:_2;
bs:labEquipmentUsed sub:_3;
bs:reagentUsed sub:_4 .
}
sub:provenance {
sub:assertion pav:createdWith ;
prov:generatedAtTime "2021-03-10T11:06:48.919028"^^xsd:dateTime;
prov:wasAttributedTo .
}
sub:pubInfo {
sub:assertion prov:wasDerivedFrom authority:3d8UhoFIEeuMmKOpLo_86Q, authority:9-iKsIFIEeuHh7NVjU9TpQ,
authority:GsYUXn7mEeuLuR-rFfCUWw, authority:h_dxNoG6EeujMV8JcmvoFg .
sub:sig npx:hasAlgorithm "RSA";
npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB";
npx:hasSignature "ZiH/Hyn7P4/i/LNmNG1bj8vMV16rIZVQvXQrryfkpcqyUshcHKwUIbbeRqJ6893PNTPVPsyOj+vyFiHRFOTHOIwbi7+yFmtvdD4kwdhc/et8MDgUGVqSo95fs1stJJ3/9Gq+dKaigG5V57DfeVhiZQiMftpaINeN/eABILJZfZ4=";
npx:hasSignatureTarget this: .
this: npx:introduces sub:step;
prov:generatedAtTime "2021-03-10T11:06:48.919028"^^xsd:dateTime;
prov:wasAttributedTo .
}