<http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w> <http://www.nanopub.org/nschema#hasAssertion> <http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w#assertion> <http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w#Head> .
<http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w> <http://www.nanopub.org/nschema#hasProvenance> <http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w#provenance> <http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w#Head> .
<http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w> <http://www.nanopub.org/nschema#hasPublicationInfo> <http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w#pubInfo> <http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w#Head> .
<http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://www.nanopub.org/nschema#Nanopublication> <http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w#Head> .
<http://purl.obolibrary.org/obo/CHEBI_17824> <http://www.w3.org/2000/01/rdf-schema#label> "isopropanol" <http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w#assertion> .
<http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w#_1> <http://www.w3.org/2000/01/rdf-schema#label> "clear supernatant" <http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w#assertion> .
<http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w#_2> <http://www.w3.org/2000/01/rdf-schema#label> "clear supernatant" <http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w#assertion> .
<http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w#step> <http://purl.org/dc/elements/1.1/description> "Transfer the very clear supernatant to a fresh tube , add two fold volume cold  isopropanol and keep it for 1 hour at -20º celcius" <http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w#assertion> .
<http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w#step> <http://purl.org/net/SMARTprotocol#hasExperimentalInput> <http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w#_1> <http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w#assertion> .
<http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w#step> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://purl.org/net/p-plan#Step> <http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w#assertion> .
<http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w#step> <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#bioSampleUsed> <http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w#_2> <http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w#assertion> .
<http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w#step> <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#labEquipmentUsed> <http://www.bioassayontology.org/bao#BAO_0010014> <http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w#assertion> .
<http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w#step> <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#reagentUsed> <http://purl.obolibrary.org/obo/CHEBI_17824> <http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w#assertion> .
<http://www.bioassayontology.org/bao#BAO_0010014> <http://www.w3.org/2000/01/rdf-schema#label> "tube" <http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w#assertion> .
<http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w#assertion> <http://purl.org/pav/createdWith> <https://nanotate.bitsfetch.com> <http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w#provenance> .
<http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w#assertion> <http://www.w3.org/ns/prov#generatedAtTime> "2021-03-10T10:40:34.285030"^^<http://www.w3.org/2001/XMLSchema#dateTime> <http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w#provenance> .
<http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w#assertion> <http://www.w3.org/ns/prov#wasAttributedTo> <https://hypothes.is/users/alejandra.sandoval> <http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w#provenance> .
<http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w#assertion> <http://www.w3.org/ns/prov#wasDerivedFrom> <https://hypothes.is/a/1AI99nJPEeuV0jNgsqleMQ> <http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w#pubInfo> .
<http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w#assertion> <http://www.w3.org/ns/prov#wasDerivedFrom> <https://hypothes.is/a/3_u-coG2EeuImTcXZHRdwA> <http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w#pubInfo> .
<http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w#assertion> <http://www.w3.org/ns/prov#wasDerivedFrom> <https://hypothes.is/a/FPj_NHJQEeusgDdsyq658w> <http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w#pubInfo> .
<http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w#assertion> <http://www.w3.org/ns/prov#wasDerivedFrom> <https://hypothes.is/a/Lh_nmHJQEeuT8WfgFIk0fw> <http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w#pubInfo> .
<http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w#sig> <http://purl.org/nanopub/x/hasAlgorithm> "RSA" <http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w#pubInfo> .
<http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w#sig> <http://purl.org/nanopub/x/hasPublicKey> "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB" <http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w#pubInfo> .
<http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w#sig> <http://purl.org/nanopub/x/hasSignature> "U1qf30RAXfxn1/xwTczDFhzx1wBXAs8h4UBAJ4OiTXKiqKhZXKRqbrholoeYD6OmZ8aEf4Z8QLIMPo2jO/5HtS6Sb81GV/WhsNeoMxYp7eJsEDJiVIeR2Qz8dq3xJnwLVwt6IK6cj57brAhPPZCYiv5YUR8oJ4DkZKpv4zV+YVk=" <http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w#pubInfo> .
<http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w#sig> <http://purl.org/nanopub/x/hasSignatureTarget> <http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w> <http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w#pubInfo> .
<http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w> <http://purl.org/nanopub/x/introduces> <http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w#step> <http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w#pubInfo> .
<http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w> <http://www.w3.org/ns/prov#generatedAtTime> "2021-03-10T10:40:34.285030"^^<http://www.w3.org/2001/XMLSchema#dateTime> <http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w#pubInfo> .
<http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w> <http://www.w3.org/ns/prov#wasAttributedTo> <https://hypothes.is/users/alejandra.sandoval> <http://purl.org/np/RAE-pu7bV-81-unFan3uVz0T16Cmsj32A6sp5fqV8LL_w#pubInfo> .