@prefix obo: .
@prefix dce: .
@prefix this: .
@prefix sub: .
@prefix authority: .
@prefix bs: .
@prefix np: .
@prefix prov: .
@prefix rdfs: .
@prefix xsd: .
@prefix npx: .
sub:Head {
this: np:hasAssertion sub:assertion;
np:hasProvenance sub:provenance;
np:hasPublicationInfo sub:pubInfo;
a np:Nanopublication .
sub:assertion rdfs:member sub:step .
}
sub:assertion {
obo:CHEBI_18153 rdfs:label "ethylene carbonate" .
obo:CHEBI_29362 rdfs:label "ethylene carbonate" .
obo:CHEBI_41609 rdfs:label "ethylene carbonate" .
sub:step dce:description "DNA point accumulation for imaging in nanoscale topography (DNA-PAINT) facilitates multiplexing in superresolution microscopy but is practically limited by slow imaging speed. We have developed DNA-PAINT-ERS, where E=ethylene carbonate, R=repeating sequence, and S=spacer, for fast and multiplexed superresolution imaging with DNA-PAINT. Here we describe detailed procedures for DNA-PAINT-ERS including reagent preparation, sample labeling, as well as image acqusition and analysis.";
bs:bioSampleUsed "microscopy", obo:CHEBI_18153, obo:CHEBI_29362, obo:CHEBI_41609;
bs:reagentUsed obo:CHEBI_18153, obo:CHEBI_29362, obo:CHEBI_41609 .
}
sub:provenance {
sub:assertion prov:generatedAtTime "2020-09-26T04:25:15.310171+00:00"^^xsd:dateTime;
prov:wasDerivedFrom authority:Rq3gcP-wEeqPmLtbnUDy2A, authority:icECav-xEeqGzeOtUkOIRw,
authority:ok_85v-xEeqsfZ9V8yVNxg .
}
sub:pubInfo {
this: prov:generatedAtTime "2020-09-26T04:25:15.310171+00:00"^^xsd:dateTime;
prov:wasAttributedTo .
sub:sig npx:hasAlgorithm "RSA";
npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCAYBVNwcjF5IK+z2KRM4MqV5gG0zVrjoqZtICaimdnS2jGy8YCweNDHUD6ZWfv5p4dWQal1NAMUm1GRXJGwGYIi3ug/zL5YLU72QeDa1ziLjWa2UHHhdGzjoZy+TB87Zhe8LI4fJUXhU+jMwxCG2O613mXmmOpNQBGFJL6+VOyWwIDAQAB";
npx:hasSignature "CZXKmRwXowx9wotw1ylvUjAag63LsoYKjW558pNwKyV1LewFNR9kjaj4fkf06XgumSJwD2gsWU900LHZ9JVT3EBKJaMPvJ5IUBHGEXsE/vRLEDD4+XEDvsQIDQ/j70ql9FRpTtLatEkCeITiVIrPtkP6KDJozMHY/reAVR+dLgo=";
npx:hasSignatureTarget this: .
}