@prefix obo: <
http://purl.obolibrary.org/obo/
> .
@prefix dce: <
http://purl.org/dc/elements/1.1/
> .
@prefix pplan: <
http://purl.org/net/p-plan#
> .
@prefix this: <
http://purl.org/np/RAHi9L2Ftk2pQ_lSeWbu23B7opN3IajkEAm8CAs35q7eE
> .
@prefix sub: <
http://purl.org/np/RAHi9L2Ftk2pQ_lSeWbu23B7opN3IajkEAm8CAs35q7eE#
> .
@prefix bs: <
https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#
> .
@prefix np: <
http://www.nanopub.org/nschema#
> .
@prefix pav: <
http://purl.org/pav/
> .
@prefix authority: <
https://hypothes.is/a/
> .
@prefix npx: <
http://purl.org/nanopub/x/
> .
@prefix xsd: <
http://www.w3.org/2001/XMLSchema#
> .
@prefix rdfs: <
http://www.w3.org/2000/01/rdf-schema#
> .
@prefix prov: <
http://www.w3.org/ns/prov#
> .
sub:Head
{
this:
np:hasAssertion
sub:assertion
;
np:hasProvenance
sub:provenance
;
np:hasPublicationInfo
sub:pubInfo
;
a
np:Nanopublication
.
}
sub:assertion
{
obo:OBI_0400155
rdfs:label
"water bath" .
sub:_1
rdfs:label
"100 mg/ml lysozyme solution" .
sub:step
dce:description
"""Bring down temperature of suspension to ~30 °C and add 20 µl of 100 mg/ml lysozyme solution (final concentration, 2 mg/ml) to it. Incubate suspension at 37 °C for 6 h to overnight in a water bath.
Note: In case of fast-growing mycobacteria such as Mycobacterium smegmatis (M. smegmatis), go directly to step 7. However, if DNA is to be isolated from slow-growing species, follow steps 4-6 which involve lysis of cells by a physical method. Because of enhanced disruption of cells, this would significantly enhance yield.""" ;
a
pplan:Step
;
bs:bioSampleUsed
<
http://www.bioassayontology.org/bao#BAO_0002104
> ;
bs:labEquipmentUsed
obo:OBI_0400155
;
bs:reagentUsed
sub:_1
.
<
http://www.bioassayontology.org/bao#BAO_0002104
>
rdfs:label
"suspension" .
}
sub:provenance
{
sub:assertion
pav:createdWith
<
https://nanotate.bitsfetch.com
> ;
prov:generatedAtTime
"2021-03-20T05:39:47.164703"^^
xsd:dateTime
;
prov:wasAttributedTo
<
https://hypothes.is/users/iannotate
> .
}
sub:pubInfo
{
sub:assertion
prov:wasDerivedFrom
authority:G3LVuojzEeu_bQeA7ItEzQ
,
authority:MVp9QojzEeuIwvdaiT_htQ
,
authority:WyamZIjzEeuUay-2mYqjfg
,
authority:cyo8HIjzEeuG1Gc-5ZGJtg
.
sub:sig
npx:hasAlgorithm
"RSA" ;
npx:hasPublicKey
"MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB" ;
npx:hasSignature
"b8dX4Z3HdbViHuM7OrEfmaDCOtdttLtr0ZLCbapt3HsT2wCBkRZ65uSCufE0VO/yZt5W9VvyI/26TGvRxlN7uBve1+r+YhPi17vQ947pXt/+FAxFBzxNoKC4mbxyKjm/0hii0UxzGH62G1dt/hwSzKzG5maO9o+GasJWm+v4psI=" ;
npx:hasSignatureTarget
this:
.
this:
npx:introduces
sub:step
;
prov:generatedAtTime
"2021-03-20T05:39:47.164703"^^
xsd:dateTime
;
prov:wasAttributedTo
<
https://hypothes.is/users/iannotate
> .
}