@prefix obo: <
http://purl.obolibrary.org/obo/
> .
@prefix dce: <
http://purl.org/dc/elements/1.1/
> .
@prefix pplan: <
http://purl.org/net/p-plan#
> .
@prefix this: <
http://purl.org/np/RAKKxFxRSyX-ofmSyQe3wzjveXXPLLQzSJBm45PDDriqQ
> .
@prefix sub: <
http://purl.org/np/RAKKxFxRSyX-ofmSyQe3wzjveXXPLLQzSJBm45PDDriqQ#
> .
@prefix bs: <
https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#
> .
@prefix np: <
http://www.nanopub.org/nschema#
> .
@prefix pav: <
http://purl.org/pav/
> .
@prefix authority: <
https://hypothes.is/a/
> .
@prefix npx: <
http://purl.org/nanopub/x/
> .
@prefix xsd: <
http://www.w3.org/2001/XMLSchema#
> .
@prefix rdfs: <
http://www.w3.org/2000/01/rdf-schema#
> .
@prefix prov: <
http://www.w3.org/ns/prov#
> .
sub:Head
{
this:
np:hasAssertion
sub:assertion
;
np:hasProvenance
sub:provenance
;
np:hasPublicationInfo
sub:pubInfo
;
a
np:Nanopublication
.
}
sub:assertion
{
obo:CHEBI_26710
rdfs:label
"NaCl" .
sub:_1
rdfs:label
"supernatant" .
sub:_2
rdfs:label
"RNAse A" .
sub:_3
rdfs:label
"chloroform: isoamyl alcohol" .
sub:step
dce:description
"After RNAse A treatment, add one volume of chloroform: isoamyl alcohol again and mix by inversions for 5 min. The tube is centrifuged for 10 min at 5000 × g at 4°C. Transfer the clear supernatant into new 2ml tube and add half volume of 5 M NaCl to the sample and mix gently by inversions." ;
a
pplan:Step
;
bs:bioSampleUsed
sub:_1
;
bs:labEquipmentUsed
<
http://www.bioassayontology.org/bao#BAO_0010014
> ;
bs:reagentUsed
obo:CHEBI_26710
,
sub:_2
,
sub:_3
.
<
http://www.bioassayontology.org/bao#BAO_0010014
>
rdfs:label
"tube" .
}
sub:provenance
{
sub:assertion
pav:createdWith
<
https://nanotate.bitsfetch.com
> ;
prov:generatedAtTime
"2021-03-18T16:08:37.156655"^^
xsd:dateTime
;
prov:wasAttributedTo
<
https://hypothes.is/users/zuluaga.lizzz
> .
}
sub:pubInfo
{
sub:assertion
prov:wasDerivedFrom
authority:5Y6WEHetEeuHODMQ_uavRg
,
authority:UWlV4netEeuPjz-MC5DRcQ
,
authority:ZDicFHetEeujJZMQKn2jcA
,
authority:dxW3QHetEeuk4qdeznt6Kg
,
authority:jh_usHetEeuphLPMcTw5HA
,
authority:sA79_netEeuXTIsjwlxzrw
.
sub:sig
npx:hasAlgorithm
"RSA" ;
npx:hasPublicKey
"MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB" ;
npx:hasSignature
"Km3e4ppAF3x/HFhFwy+upjcmVLBHT+LxVvzqhN0BoNSKzt+4kYRZ1IWWDD7Ur1qBOk9mDN7EYbWIFp6dWzgOYXueGEqnciSIJ/CSHt0SUWE9FYE+JaS08vu9GilvbRsMPusCUxRpnp2W3NP4m+f2NhOfZA0Z8Z37q0UMXhlWGGo=" ;
npx:hasSignatureTarget
this:
.
this:
npx:introduces
sub:step
;
prov:generatedAtTime
"2021-03-18T16:08:37.156655"^^
xsd:dateTime
;
prov:wasAttributedTo
<
https://hypothes.is/users/zuluaga.lizzz
> .
}