@prefix obo: <
http://purl.obolibrary.org/obo/
> .
@prefix dce: <
http://purl.org/dc/elements/1.1/
> .
@prefix pplan: <
http://purl.org/net/p-plan#
> .
@prefix this: <
http://purl.org/np/RAKzzNvwKd7CCCX7I-7_BjKcZ7yyC9qoZiNar5Ly0RfIU
> .
@prefix sub: <
http://purl.org/np/RAKzzNvwKd7CCCX7I-7_BjKcZ7yyC9qoZiNar5Ly0RfIU#
> .
@prefix bs: <
https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#
> .
@prefix np: <
http://www.nanopub.org/nschema#
> .
@prefix pav: <
http://purl.org/pav/
> .
@prefix authority: <
https://hypothes.is/a/
> .
@prefix npx: <
http://purl.org/nanopub/x/
> .
@prefix xsd: <
http://www.w3.org/2001/XMLSchema#
> .
@prefix rdfs: <
http://www.w3.org/2000/01/rdf-schema#
> .
@prefix prov: <
http://www.w3.org/ns/prov#
> .
sub:Head
{
this:
np:hasAssertion
sub:assertion
;
np:hasProvenance
sub:provenance
;
np:hasPublicationInfo
sub:pubInfo
;
a
np:Nanopublication
.
}
sub:assertion
{
obo:OBI_0000918
rdfs:label
"DMEM" .
sub:_1
rdfs:label
"cells" .
sub:step
dce:description
"""Wash cells (centrifuge at 100 x g for 10 min) with 10 ml of sterile DMEM (without FBS) twice.
Note: Cold DMEM is not used routinely for washing lymphocytes from culture cavities while setting up cultures. Rather when monocytes bound tightly to plastic cavities are needed to be harvested pre-chilled DMEM can be used.""" ;
a
pplan:Step
;
bs:bioSampleUsed
sub:_1
;
bs:reagentUsed
obo:OBI_0000918
.
}
sub:provenance
{
sub:assertion
pav:createdWith
<
https://nanotate.bitsfetch.com
> ;
prov:generatedAtTime
"2021-02-14T05:27:45.411427"^^
xsd:dateTime
;
prov:wasAttributedTo
<
https://hypothes.is/users/zuluaga.lizzz
> .
}
sub:pubInfo
{
sub:assertion
prov:wasDerivedFrom
authority:0GCTfm6sEeuTj4ssi2Skaw
,
authority:5bafwG6sEeuROrODpsG-8g
,
authority:A5OGZm6tEeuYVk-uglPVIA
.
sub:sig
npx:hasAlgorithm
"RSA" ;
npx:hasPublicKey
"MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB" ;
npx:hasSignature
"ba6UiZ8V8p6gOUif5M2DANaOaIcViKw+nkGyxzsJ6OJK3vZnWIAYKjHN4RsJXjzKzMuX+mqaraJnAS3EeZ9b6eXUuvLKfVOXWc0wowgXbhkXegvMbjgDhBQrvf3qbqT1YpPTasRgEB/+GMXhgSmANANz+vVbyH4wIXNDN9DdBMo=" ;
npx:hasSignatureTarget
this:
.
this:
npx:introduces
sub:step
;
prov:generatedAtTime
"2021-02-14T05:27:45.411427"^^
xsd:dateTime
;
prov:wasAttributedTo
<
https://hypothes.is/users/zuluaga.lizzz
> .
}