@prefix dce: .
@prefix pplan: .
@prefix this: .
@prefix sub: .
@prefix bs: .
@prefix np: .
@prefix pav: .
@prefix authority: .
@prefix npx: .
@prefix xsd: .
@prefix rdfs: .
@prefix prov: .
@prefix sp: .
sub:Head {
this: np:hasAssertion sub:assertion;
np:hasProvenance sub:provenance;
np:hasPublicationInfo sub:pubInfo;
a np:Nanopublication .
}
sub:assertion {
sub:_1 rdfs:label "mosquitoes" .
sub:_10 rdfs:label "1% SDS" .
sub:_2 rdfs:label "1% Triton -X" .
sub:_3 rdfs:label "mosquitoes" .
sub:_4 rdfs:label "micropistle" .
sub:_5 rdfs:label "1.5 ml eppendorf tube" .
sub:_6 rdfs:label "1X STE buffer" .
sub:_7 rdfs:label "Proteinase K (20mg/ml)" .
sub:_8 rdfs:label "100mM sucrose" .
sub:_9 rdfs:label "Rnase A (20mg/ml)" .
sub:step dce:description "Grind mosquitoes in 1.5 ml eppendorf tube with micropistle in 50-100 µl 1X STE buffer ( 50mM Nacl,50mM Tris- HCL,100mM EDTA, Ph 8.0) along with 100mM sucrose. Add 1X STE buffer to a total volume of 300-500 µl for a single mosquito and 1 ml for mosquito pool like 4,6,8,10 numbers. Then add 1% SDS ,1% Triton -X , 10 µl/ ml Rnase A (20mg/ml), 20 µl/ ml Proteinase K (20mg/ml) and mix it.";
sp:hasExperimentalInput sub:_1;
a pplan:Step;
bs:bioSampleUsed sub:_3;
bs:labEquipmentUsed sub:_4, sub:_5;
bs:reagentUsed sub:_10, sub:_2, sub:_6, sub:_7, sub:_8, sub:_9 .
}
sub:provenance {
sub:assertion pav:createdWith ;
prov:generatedAtTime "2021-03-10T10:33:19.355370"^^xsd:dateTime;
prov:wasAttributedTo .
}
sub:pubInfo {
sub:assertion prov:wasDerivedFrom authority:8b4bAHJNEeu01RN1eV0q-Q, authority:ESqevnJOEeu1UX9lnqErIQ,
authority:IrIzfHJOEeujx7t3IvpXzA, authority:OkmgznJOEeudNEPmE26k-Q, authority:UBFH9HJOEeusy1eAko5oQw,
authority:Usa7nHJNEeuoHBdiYKYtlg, authority:cWBYjnJMEeuOKD-DjOC3Cw, authority:drQGkHJNEeudQGs6yWujow,
authority:lG67TnJNEeu01GMwoWiMRA, authority:rawtGHJMEeu0J4cNJ9O11g .
sub:sig npx:hasAlgorithm "RSA";
npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB";
npx:hasSignature "Kd4Aa6fDdpMWhH77AgLNFo/GE7w50Y0bXMfwIOIVdP+2Jp7DcBZ3K28i9HOzqkwNBC9YZmAKwLlwHp818FZw1VIUbdMpPIaCSwdAsSJgsv/Ov/vuO6093NjHOignVj4QsE9+XO9WZOyFBX1XxDAbO2anaVMuoEQa4HYFefmlDOc=";
npx:hasSignatureTarget this: .
this: npx:introduces sub:step;
prov:generatedAtTime "2021-03-10T10:33:19.355370"^^xsd:dateTime;
prov:wasAttributedTo .
}