http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY#Head
http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY
http://www.nanopub.org/nschema#hasAssertion
http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY#assertion
http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY
http://www.nanopub.org/nschema#hasProvenance
http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY#provenance
http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY
http://www.nanopub.org/nschema#hasPublicationInfo
http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY#pubInfo
http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY
http://www.w3.org/1999/02/22-rdf-syntax-ns#type
http://www.nanopub.org/nschema#Nanopublication
http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY#assertion
http://purl.obolibrary.org/obo/CHEBI_26710
http://www.w3.org/2000/01/rdf-schema#label
5 M NaCl
http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY#_1
http://www.w3.org/2000/01/rdf-schema#label
supernatant
http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY#_2
http://www.w3.org/2000/01/rdf-schema#label
chloroform: isoamyl alcohol
http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY#_3
http://www.w3.org/2000/01/rdf-schema#label
RNAse A
http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY#step
http://purl.org/dc/elements/1.1/description
After RNAse A treatment, add one volume of chloroform: isoamyl alcohol again and mix by inversions for 5 min. The tube is centrifuged for 10 min at 5000 × g at 4°C. Transfer the clear supernatant into new 2ml tube and add half volume of 5 M NaCl to the sample and mix gently by inversions.
http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY#step
http://www.w3.org/1999/02/22-rdf-syntax-ns#type
http://purl.org/net/p-plan#Step
http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY#step
https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#bioSampleUsed
http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY#_1
http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY#step
https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#labEquipmentUsed
http://www.bioassayontology.org/bao#BAO_0010014
http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY#step
https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#reagentUsed
http://purl.obolibrary.org/obo/CHEBI_26710
http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY#step
https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#reagentUsed
http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY#_2
http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY#step
https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#reagentUsed
http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY#_3
http://www.bioassayontology.org/bao#BAO_0010014
http://www.w3.org/2000/01/rdf-schema#label
tube
http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY#provenance
http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY#assertion
http://purl.org/pav/createdWith
https://nanotate.bitsfetch.com
http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY#assertion
http://www.w3.org/ns/prov#generatedAtTime
2021-03-11T16:42:19.939476
http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY#assertion
http://www.w3.org/ns/prov#wasAttributedTo
https://hypothes.is/users/iannotate
http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY#pubInfo
http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY#assertion
http://www.w3.org/ns/prov#wasDerivedFrom
https://hypothes.is/a/65gnrIKwEeu8ozv-JryAeQ
http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY#assertion
http://www.w3.org/ns/prov#wasDerivedFrom
https://hypothes.is/a/E7j6IoKxEeuOsU_XwyDh1w
http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY#assertion
http://www.w3.org/ns/prov#wasDerivedFrom
https://hypothes.is/a/LAccdoKxEeuiRiOOl5XFJA
http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY#assertion
http://www.w3.org/ns/prov#wasDerivedFrom
https://hypothes.is/a/SUBJ6IKxEeuOTeteBAH1mw
http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY#assertion
http://www.w3.org/ns/prov#wasDerivedFrom
https://hypothes.is/a/_rt9SIKwEeuOS7PRsXqlQA
http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY#assertion
http://www.w3.org/ns/prov#wasDerivedFrom
https://hypothes.is/a/nLN1sIKwEeufn8eMqZYi6g
http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY#sig
http://purl.org/nanopub/x/hasAlgorithm
RSA
http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY#sig
http://purl.org/nanopub/x/hasPublicKey
MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB
http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY#sig
http://purl.org/nanopub/x/hasSignature
cCe5S4PTfHVNKbJDNVTTQAqxqGXwmCdNl2CL+z9K1r5knTrma1JscEMcqbOvXXiK6IXqUJ8SJ22C1RFBjxCLccSsoInb79UamARsI2c6Zh248oaxNEsSHF8BKLmWGwwDTKEZ1oqpZ7j4igSflkPVAUQ06IgSP1s6STvxRc3jNJs=
http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY#sig
http://purl.org/nanopub/x/hasSignatureTarget
http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY
http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY
http://purl.org/nanopub/x/introduces
http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY#step
http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY
http://www.w3.org/ns/prov#generatedAtTime
2021-03-11T16:42:19.939476
http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY
http://www.w3.org/ns/prov#wasAttributedTo
https://hypothes.is/users/iannotate