@prefix pplan: <http://purl.org/net/p-plan#> .
@prefix dce: <http://purl.org/dc/elements/1.1/> .
@prefix this: <http://purl.org/np/RAXplccK-fzdrl3kIsznNXr7k5gu8qJsyWLiom8B-xi8M> .
@prefix sub: <http://purl.org/np/RAXplccK-fzdrl3kIsznNXr7k5gu8qJsyWLiom8B-xi8M#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix sp: <http://purl.org/net/SMARTprotocol#> .

sub:Head {
  this: np:hasAssertion sub:assertion;
    np:hasProvenance sub:provenance;
    np:hasPublicationInfo sub:pubInfo;
    a np:Nanopublication .
}

sub:assertion {
  <http://purl.obolibrary.org/obo/UBERON_0036018> rdfs:label "pellet " .
  
  sub:_1 rdfs:label "autoclaved water" .
  
  sub:_2 rdfs:label "RNase free water" .
  
  sub:step dce:description "Dissolve the pellet in 20-30 µl of RNase free water (commercial) or autoclaved water.";
    sp:hasExperimentalInput <http://purl.obolibrary.org/obo/UBERON_0036018>;
    a pplan:Step;
    bs:bioSampleUsed <http://purl.obolibrary.org/obo/UBERON_0036018>;
    bs:reagentUsed sub:_1, sub:_2 .
}

sub:provenance {
  sub:assertion pav:createdWith <https://nanotate.bitsfetch.com>;
    prov:generatedAtTime "2021-03-10T10:18:43.473022"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/alejandra.sandoval> .
}

sub:pubInfo {
  sub:assertion prov:wasDerivedFrom authority:51wNHnJKEeuZF_P4cM-i3Q, authority:C80o1nJLEeuZGY97TWn4Ag,
      authority:ISewtnJLEeuYbUtcxpuF7w, authority:N2nSjHJLEeuejA8Ek9tG5A .
  
  sub:sig npx:hasAlgorithm "RSA";
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB";
    npx:hasSignature "OfydTQ1QRpJ9Fx9AY3z4U7QqVGd8mPY5R8B7ES+XdeHMmmtyAuZDYtHk0qoYovyaPGXIXEwxwht9RsYKG7EvwzyO+WkcRH0TenG6/G+nW5VFr2comSzY6YlzT/drAIPQ1RpNFikcjCqkrAvDlHmWjzsIk6qEtmBwODpqidw8ALk=";
    npx:hasSignatureTarget this: .
  
  this: npx:introduces sub:step;
    prov:generatedAtTime "2021-03-10T10:18:43.473022"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/alejandra.sandoval> .
}