@prefix dce: <
http://purl.org/dc/elements/1.1/
> .
@prefix pplan: <
http://purl.org/net/p-plan#
> .
@prefix this: <
http://purl.org/np/RAfk1ft_CjnFbKMJWcpUxgTwf8I6lf4MFOpO6trl2V9a0
> .
@prefix sub: <
http://purl.org/np/RAfk1ft_CjnFbKMJWcpUxgTwf8I6lf4MFOpO6trl2V9a0#
> .
@prefix bs: <
https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#
> .
@prefix np: <
http://www.nanopub.org/nschema#
> .
@prefix pav: <
http://purl.org/pav/
> .
@prefix authority: <
https://hypothes.is/a/
> .
@prefix npx: <
http://purl.org/nanopub/x/
> .
@prefix xsd: <
http://www.w3.org/2001/XMLSchema#
> .
@prefix rdfs: <
http://www.w3.org/2000/01/rdf-schema#
> .
@prefix prov: <
http://www.w3.org/ns/prov#
> .
sub:Head
{
this:
np:hasAssertion
sub:assertion
;
np:hasProvenance
sub:provenance
;
np:hasPublicationInfo
sub:pubInfo
;
a
np:Nanopublication
.
}
sub:assertion
{
sub:_1
rdfs:label
"swing-out bucket" .
sub:_2
rdfs:label
"tubes" .
sub:step
dce:description
"Centrifuge the tubes (without any delay) for 30 min at 100 x g in 4 °C in a swing-out bucket. Fixed angle rotors also can be used but would require more caution when separating cells in interphase." ;
a
pplan:Step
;
bs:labEquipmentUsed
sub:_1
,
sub:_2
.
}
sub:provenance
{
sub:assertion
pav:createdWith
<
https://nanotate.bitsfetch.com
> ;
prov:generatedAtTime
"2021-03-22T16:50:45.625023"^^
xsd:dateTime
;
prov:wasAttributedTo
<
https://hypothes.is/users/alejandra.sandoval
> .
}
sub:pubInfo
{
sub:assertion
prov:wasDerivedFrom
authority:idzKhotXEeudGn84TW8yBQ
,
authority:lldI_otXEeufaj9ipczYUw
,
authority:pgS7TItXEeuue8uxHjnLig
.
sub:sig
npx:hasAlgorithm
"RSA" ;
npx:hasPublicKey
"MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB" ;
npx:hasSignature
"KOYCRoDGn1CXUXw7HMWNRHePFwWz7ZkymuuA+4r27RgchwWv9RdR//CougOLdYCC1ClbPUYEJIDUFih2BgNmmKAMNm3wUdow3tN2KU+P/uKuhI+E+/4ZTgqzB3YC9T6/r2uRWyVU8R+/9MHEIyT14YSPxRkjl1pHD+LInDvmWnU=" ;
npx:hasSignatureTarget
this:
.
this:
npx:introduces
sub:step
;
prov:generatedAtTime
"2021-03-22T16:50:45.625023"^^
xsd:dateTime
;
prov:wasAttributedTo
<
https://hypothes.is/users/alejandra.sandoval
> .
}