@prefix dce: <http://purl.org/dc/elements/1.1/> .
@prefix pplan: <http://purl.org/net/p-plan#> .
@prefix this: <http://purl.org/np/RAiDRLESQJ9y2mMiyiya5TvKxaN_1-MQMLyA5K0a4v9lc> .
@prefix sub: <http://purl.org/np/RAiDRLESQJ9y2mMiyiya5TvKxaN_1-MQMLyA5K0a4v9lc#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix sp: <http://purl.org/net/SMARTprotocol#> .

sub:Head {
  this: np:hasAssertion sub:assertion;
    np:hasProvenance sub:provenance;
    np:hasPublicationInfo sub:pubInfo;
    a np:Nanopublication .
}

sub:assertion {
  <http://purl.obolibrary.org/obo/CHEBI_53692> rdfs:label "histopaque" .
  
  sub:_1 rdfs:label "PBMCs" .
  
  sub:_2 rdfs:label "PBMCs" .
  
  sub:_3 rdfs:label "medium" .
  
  sub:step dce:description "Aspirate the whitish buffy coat (about 1 ml) (PBMCs) formed in the interphase between histopaque and medium.";
    sp:hasExperimentalOutput sub:_1;
    a pplan:Step;
    bs:bioSampleUsed sub:_2;
    bs:reagentUsed <http://purl.obolibrary.org/obo/CHEBI_53692>, sub:_3 .
}

sub:provenance {
  sub:assertion pav:createdWith <https://nanotate.bitsfetch.com>;
    prov:generatedAtTime "2021-03-04T15:10:07.828515"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/iannotate> .
}

sub:pubInfo {
  sub:assertion prov:wasDerivedFrom authority:UAdOYn0kEeuxvTfs_TYV5w, authority:bKsckn0kEeuonlunG0SdfA,
      authority:e0rt_n0kEeuxuUs5o5z_ew, authority:xEyXOH0jEeuWrSfb5XYXCg .
  
  sub:sig npx:hasAlgorithm "RSA";
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB";
    npx:hasSignature "WxVNwbZWYrAiBLDXgvC8T2znn/l8A0d4whPkCw3uvVdPAPVQK156+eE8eZGD+gBa8Zcqw2Z8coHNeT1xXDIqbADvjv8skIE6tJp9KhAcGeK3vLn3wCM+QClcRbm3wC0Ay9csI3W5dGJs3njRa67EDC63z4Id4UUQCCIBKC2yogc=";
    npx:hasSignatureTarget this: .
  
  this: npx:introduces sub:step;
    prov:generatedAtTime "2021-03-04T15:10:07.828515"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/iannotate> .
}