@prefix dce: <http://purl.org/dc/elements/1.1/> .
@prefix pplan: <http://purl.org/net/p-plan#> .
@prefix this: <http://purl.org/np/RAjLh4dT8yHvxgoFfivNwEdYFYMolH8BCvHNOub1kFE7E> .
@prefix sub: <http://purl.org/np/RAjLh4dT8yHvxgoFfivNwEdYFYMolH8BCvHNOub1kFE7E#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix sp: <http://purl.org/net/SMARTprotocol#> .
sub:Head {
   this: np:hasAssertion sub:assertion ;
    np:hasProvenance sub:provenance ;
    np:hasPublicationInfo sub:pubInfo ;
    a np:Nanopublication .
}
sub:assertion {
   sp:ChloroformIsoamylAlcoholMixture rdfs:label "chloroform:isoamyl alcohol" .
  sub:step dce:description "Repeat the above step(5) , then add chloroform:isoamyl alcohol(24:1) and centrifuge at 12,000g for 10 minutes at  4º celcius  ." ;
    a pplan:Step ;
    bs:reagentUsed sp:ChloroformIsoamylAlcoholMixture .
}
sub:provenance {
   sub:assertion pav:createdWith <https://nanotate.bitsfetch.com> ;
    prov:generatedAtTime "2021-03-11T10:49:52.989941"^^xsd:dateTime ;
    prov:wasAttributedTo <https://hypothes.is/users/iannotate> .
}
sub:pubInfo {
   sub:assertion prov:wasDerivedFrom authority:ZtWrQoJ_Eeuy9q-Bm7AsEA , authority:gBHnOIJ_EeuMEtcNHtvLdw .
  sub:sig npx:hasAlgorithm "RSA" ;
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB" ;
    npx:hasSignature "NorQkTi1BP763XXafT4B4GMfXcL2SooVLomsfjkT2FrTnKiyf6yzGRnHCsNP94FUv/TKtkycH6yski4KRKRaZ08k/GeknHnkDIDfaUs3lVnPGtMSNZUSGsvhRTqHnquXMNRFnJHny5DbNbreiBjsKZlD2tDqKUHk8z5j+9JTTvw=" ;
    npx:hasSignatureTarget this: .
  this: npx:introduces sub:step ;
    prov:generatedAtTime "2021-03-11T10:49:52.989941"^^xsd:dateTime ;
    prov:wasAttributedTo <https://hypothes.is/users/iannotate> .
}