@prefix obo: .
@prefix dce: .
@prefix this: .
@prefix sub: .
@prefix authority: .
@prefix bs: .
@prefix np: .
@prefix prov: .
@prefix rdfs: .
@prefix sp: .
@prefix xsd: .
@prefix npx: .
sub:Head {
this: np:hasAssertion sub:assertion;
np:hasProvenance sub:provenance;
np:hasPublicationInfo sub:pubInfo;
a np:Nanopublication .
sub:assertion rdfs:member sub:step .
}
sub:assertion {
obo:CHEBI_29362 rdfs:label "ethylene carbonate" .
obo:CHEBI_35225 rdfs:label "imaging buffer" .
sub:step dce:description "Add imaging buffer with desired ratios of Buffer C (500 mM), ethylene carbonate, and IS-CF660R at 1-2 nM final concentration. The exact concentration of IS may need to be adjusted depending on the target and based on the imaging kinetics.";
sp:hasExperimentalInput obo:CHEBI_29362;
bs:bioSampleUsed obo:CHEBI_35225 .
}
sub:provenance {
sub:assertion prov:generatedAtTime "2020-09-27T00:50:41.265044+00:00"^^xsd:dateTime;
prov:wasDerivedFrom authority:7Z1sugBbEeu9_wtvk1iAjw, authority:FrhgjABcEeu5B4dnXgvb_A,
authority:d5JrdABbEeuatj9X3vvoXw .
}
sub:pubInfo {
this: prov:generatedAtTime "2020-09-27T00:50:41.265044+00:00"^^xsd:dateTime;
prov:wasAttributedTo .
sub:sig npx:hasAlgorithm "RSA";
npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCAYBVNwcjF5IK+z2KRM4MqV5gG0zVrjoqZtICaimdnS2jGy8YCweNDHUD6ZWfv5p4dWQal1NAMUm1GRXJGwGYIi3ug/zL5YLU72QeDa1ziLjWa2UHHhdGzjoZy+TB87Zhe8LI4fJUXhU+jMwxCG2O613mXmmOpNQBGFJL6+VOyWwIDAQAB";
npx:hasSignature "IRswte1hL0JgEyB3cvL5eRwVC4liF/61G6aO9MVZk1Nbb4+lrVQ3md++eLmtW9TZg/lRkqsdaQhL0DJUqD/pifSBf5TFB8JAt4RSt2V3TzkcxWlk5QaGmYSJDjJ3JdCr9yLXXd6klmKhLtr80tRqNHvhDZigjDb1PgW35fgGjT8=";
npx:hasSignatureTarget this: .
}