http://purl.org/np/RAqSdY5sD_93cw3lbnYepanGTewPYhdudnfjleS0iUVls#Head
http://purl.org/np/RAqSdY5sD_93cw3lbnYepanGTewPYhdudnfjleS0iUVls
http://www.nanopub.org/nschema#hasAssertion
http://purl.org/np/RAqSdY5sD_93cw3lbnYepanGTewPYhdudnfjleS0iUVls#assertion
http://purl.org/np/RAqSdY5sD_93cw3lbnYepanGTewPYhdudnfjleS0iUVls
http://www.nanopub.org/nschema#hasProvenance
http://purl.org/np/RAqSdY5sD_93cw3lbnYepanGTewPYhdudnfjleS0iUVls#provenance
http://purl.org/np/RAqSdY5sD_93cw3lbnYepanGTewPYhdudnfjleS0iUVls
http://www.nanopub.org/nschema#hasPublicationInfo
http://purl.org/np/RAqSdY5sD_93cw3lbnYepanGTewPYhdudnfjleS0iUVls#pubInfo
http://purl.org/np/RAqSdY5sD_93cw3lbnYepanGTewPYhdudnfjleS0iUVls
http://www.w3.org/1999/02/22-rdf-syntax-ns#type
http://www.nanopub.org/nschema#Nanopublication
http://purl.org/np/RAqSdY5sD_93cw3lbnYepanGTewPYhdudnfjleS0iUVls#assertion
http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C62218
http://www.w3.org/2000/01/rdf-schema#label
70% ethanol
http://purl.obolibrary.org/obo/CHEBI_16991
http://www.w3.org/2000/01/rdf-schema#label
DNA
http://purl.org/np/RAqSdY5sD_93cw3lbnYepanGTewPYhdudnfjleS0iUVls#_1
http://www.w3.org/2000/01/rdf-schema#label
pellet
http://purl.org/np/RAqSdY5sD_93cw3lbnYepanGTewPYhdudnfjleS0iUVls#_2
http://www.w3.org/2000/01/rdf-schema#label
tubes
http://purl.org/np/RAqSdY5sD_93cw3lbnYepanGTewPYhdudnfjleS0iUVls#_3
http://www.w3.org/2000/01/rdf-schema#label
TE
http://purl.org/np/RAqSdY5sD_93cw3lbnYepanGTewPYhdudnfjleS0iUVls#step
http://purl.org/dc/elements/1.1/description
After incubation, the tubes were centrifuged at 5000 × g for 10 min at 4°C and the supernatant was gently removed. The pellet is washed two times with 1ml of 70% ethanol and the DNA is pellet by 5000 × g at 4°C for only 5 min. The supernatant is discarded and the pellet is air-dried (10 min). The pellet are allowed to re-suspend in 50 μL of TE (10 mM Tris. HCl pH 8.0; 1 mM EDTA pH 8.0).
http://purl.org/np/RAqSdY5sD_93cw3lbnYepanGTewPYhdudnfjleS0iUVls#step
http://purl.org/net/SMARTprotocol#hasExperimentalOutput
http://purl.obolibrary.org/obo/CHEBI_16991
http://purl.org/np/RAqSdY5sD_93cw3lbnYepanGTewPYhdudnfjleS0iUVls#step
http://www.w3.org/1999/02/22-rdf-syntax-ns#type
http://purl.org/net/p-plan#Step
http://purl.org/np/RAqSdY5sD_93cw3lbnYepanGTewPYhdudnfjleS0iUVls#step
https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#bioSampleUsed
http://purl.obolibrary.org/obo/CHEBI_16991
http://purl.org/np/RAqSdY5sD_93cw3lbnYepanGTewPYhdudnfjleS0iUVls#step
https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#bioSampleUsed
http://purl.org/np/RAqSdY5sD_93cw3lbnYepanGTewPYhdudnfjleS0iUVls#_1
http://purl.org/np/RAqSdY5sD_93cw3lbnYepanGTewPYhdudnfjleS0iUVls#step
https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#labEquipmentUsed
http://purl.org/np/RAqSdY5sD_93cw3lbnYepanGTewPYhdudnfjleS0iUVls#_2
http://purl.org/np/RAqSdY5sD_93cw3lbnYepanGTewPYhdudnfjleS0iUVls#step
https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#reagentUsed
http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C62218
http://purl.org/np/RAqSdY5sD_93cw3lbnYepanGTewPYhdudnfjleS0iUVls#step
https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#reagentUsed
http://purl.org/np/RAqSdY5sD_93cw3lbnYepanGTewPYhdudnfjleS0iUVls#_3
http://purl.org/np/RAqSdY5sD_93cw3lbnYepanGTewPYhdudnfjleS0iUVls#provenance
http://purl.org/np/RAqSdY5sD_93cw3lbnYepanGTewPYhdudnfjleS0iUVls#assertion
http://purl.org/pav/createdWith
https://nanotate.bitsfetch.com
http://purl.org/np/RAqSdY5sD_93cw3lbnYepanGTewPYhdudnfjleS0iUVls#assertion
http://www.w3.org/ns/prov#generatedAtTime
2021-02-25T16:19:32.955991
http://purl.org/np/RAqSdY5sD_93cw3lbnYepanGTewPYhdudnfjleS0iUVls#assertion
http://www.w3.org/ns/prov#wasAttributedTo
https://hypothes.is/users/zuluaga.lizzz
http://purl.org/np/RAqSdY5sD_93cw3lbnYepanGTewPYhdudnfjleS0iUVls#pubInfo
http://purl.org/np/RAqSdY5sD_93cw3lbnYepanGTewPYhdudnfjleS0iUVls#assertion
http://www.w3.org/ns/prov#wasDerivedFrom
https://hypothes.is/a/0vwt1neuEeugLneRnV11IQ
http://purl.org/np/RAqSdY5sD_93cw3lbnYepanGTewPYhdudnfjleS0iUVls#assertion
http://www.w3.org/ns/prov#wasDerivedFrom
https://hypothes.is/a/U5V5WHeuEeuaqaeDOnia0g
http://purl.org/np/RAqSdY5sD_93cw3lbnYepanGTewPYhdudnfjleS0iUVls#assertion
http://www.w3.org/ns/prov#wasDerivedFrom
https://hypothes.is/a/Zutt3HeuEeuuZH8J3g6HLA
http://purl.org/np/RAqSdY5sD_93cw3lbnYepanGTewPYhdudnfjleS0iUVls#assertion
http://www.w3.org/ns/prov#wasDerivedFrom
https://hypothes.is/a/i38vTneuEeuNAsMsRIF24w
http://purl.org/np/RAqSdY5sD_93cw3lbnYepanGTewPYhdudnfjleS0iUVls#assertion
http://www.w3.org/ns/prov#wasDerivedFrom
https://hypothes.is/a/oNTjoneuEeu3I3Om5Ihl4A
http://purl.org/np/RAqSdY5sD_93cw3lbnYepanGTewPYhdudnfjleS0iUVls#assertion
http://www.w3.org/ns/prov#wasDerivedFrom
https://hypothes.is/a/uGfetneuEeuQLE_Q4_h28w
http://purl.org/np/RAqSdY5sD_93cw3lbnYepanGTewPYhdudnfjleS0iUVls#sig
http://purl.org/nanopub/x/hasAlgorithm
RSA
http://purl.org/np/RAqSdY5sD_93cw3lbnYepanGTewPYhdudnfjleS0iUVls#sig
http://purl.org/nanopub/x/hasPublicKey
MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB
http://purl.org/np/RAqSdY5sD_93cw3lbnYepanGTewPYhdudnfjleS0iUVls#sig
http://purl.org/nanopub/x/hasSignature
CK0mUuEnSryqDlktLiDzRtTbtqvviyX1sWeq4FLSkqK62YSYfkFnQ/HwTyKezY7DqmGU2q8fUJx2xP1NI/uAPsPyWpMoNuOkKce6F1rlz3waaMHVCHWflyKmCQyXKibwDd0VS/NSBecXujFnqAuLY0mDrXc4VtLm5xIcKrh8YTk=
http://purl.org/np/RAqSdY5sD_93cw3lbnYepanGTewPYhdudnfjleS0iUVls#sig
http://purl.org/nanopub/x/hasSignatureTarget
http://purl.org/np/RAqSdY5sD_93cw3lbnYepanGTewPYhdudnfjleS0iUVls
http://purl.org/np/RAqSdY5sD_93cw3lbnYepanGTewPYhdudnfjleS0iUVls
http://purl.org/nanopub/x/introduces
http://purl.org/np/RAqSdY5sD_93cw3lbnYepanGTewPYhdudnfjleS0iUVls#step
http://purl.org/np/RAqSdY5sD_93cw3lbnYepanGTewPYhdudnfjleS0iUVls
http://www.w3.org/ns/prov#generatedAtTime
2021-02-25T16:19:32.955991
http://purl.org/np/RAqSdY5sD_93cw3lbnYepanGTewPYhdudnfjleS0iUVls
http://www.w3.org/ns/prov#wasAttributedTo
https://hypothes.is/users/zuluaga.lizzz