@prefix dce: .
@prefix pplan: .
@prefix this: .
@prefix sub: .
@prefix bs: .
@prefix np: .
@prefix pav: .
@prefix authority: .
@prefix npx: .
@prefix xsd: .
@prefix rdfs: .
@prefix prov: .
@prefix sp: .
sub:Head {
this: np:hasAssertion sub:assertion;
np:hasProvenance sub:provenance;
np:hasPublicationInfo sub:pubInfo;
a np:Nanopublication .
}
sub:assertion {
rdfs:label "DMEM" .
sub:_1 rdfs:label "cells" .
sub:_2 rdfs:label "cells" .
sub:step dce:description """Wash cells (centrifuge at 100 x g for 10 min) with 10 ml of sterile DMEM (without FBS) twice.
Note: Cold DMEM is not used routinely for washing lymphocytes from culture cavities while setting up cultures. Rather when monocytes bound tightly to plastic cavities are needed to be harvested pre-chilled DMEM can be used.
""";
sp:hasExperimentalOutput sub:_1;
a pplan:Step;
bs:bioSampleUsed sub:_2;
bs:reagentUsed .
}
sub:provenance {
sub:assertion pav:createdWith ;
prov:generatedAtTime "2021-03-05T10:08:35.231457"^^xsd:dateTime;
prov:wasAttributedTo .
}
sub:pubInfo {
sub:assertion prov:wasDerivedFrom authority:Iqikin0oEeuEobN3tukkMQ, authority:Pn1VKn0oEeut2T99emTI0A,
authority:Ul6qxn0oEeufO-9TegxgZA .
sub:sig npx:hasAlgorithm "RSA";
npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB";
npx:hasSignature "MaVfAfiTemYOYoX4V0FCGMP6Krh46NbiDyUU2qhr2iGn4DZRQY09gJryonoByj4nc6LWXT2b6CUz4q9KKk7+952Crb9CooP7upaYJ8WKeD8OoTjdpSJoktwUpT4OnC27D1nNTadqe11Pc3DeaykZSnAx0T2TjSvlWfxE6VAzJu4=";
npx:hasSignatureTarget this: .
this: npx:introduces sub:step;
prov:generatedAtTime "2021-03-05T10:08:35.231457"^^xsd:dateTime;
prov:wasAttributedTo .
}