@prefix pplan: <http://purl.org/net/p-plan#> .
@prefix this: <http://purl.org/np/RAwrombnCGU6p1zDbDcWrOJNTrIdhwQAY7DoRSUmx7jeI> .
@prefix sub: <http://purl.org/np/RAwrombnCGU6p1zDbDcWrOJNTrIdhwQAY7DoRSUmx7jeI#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix sp: <http://purl.org/net/SMARTprotocol#> .
@prefix dc: <http://purl.org/dc/elements/1.1/> .

sub:Head {
  this: np:hasAssertion sub:assertion;
    np:hasProvenance sub:provenance;
    np:hasPublicationInfo sub:pubInfo;
    a np:Nanopublication .
}

sub:assertion {
  <http://purl.bioontology.org/ontology/NCBITAXON/2> rdfs:label "bacteria" .
  
  sub:_1 rdfs:label "pellet" .
  
  sub:_2 rdfs:label "mortar" .
  
  sub:_3 rdfs:label "pestle" .
  
  sub:_4 rdfs:label "liquid N2" .
  
  sub:step dc:description "Snap freeze pellet in liquid N2 and immediately transfer to a mortar. Using a pestle, crush bacteria while repeatedly adding liquid N2 (~20 ml) to it.";
    sp:hasExperimentalInput <http://purl.bioontology.org/ontology/NCBITAXON/2>;
    a pplan:Step;
    bs:bioSampleUsed <http://purl.bioontology.org/ontology/NCBITAXON/2>, sub:_1;
    bs:labEquipmentUsed sub:_2, sub:_3;
    bs:reagentUsed sub:_4 .
}

sub:provenance {
  sub:assertion pav:createdWith <https://nanotate.bitsfetch.com>;
    prov:generatedAtTime "2021-03-20T05:39:07.806327"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/iannotate> .
}

sub:pubInfo {
  sub:assertion prov:wasDerivedFrom authority:1pxsjojzEeu2LK-KmH1PNQ, authority:5xVFuIjzEeuhk8tL1jnOMg,
      authority:9x1qTojzEeuU-Y-KQSQvkA, authority:ERersoj0EeuSRJvpmvsAnA, authority:r1SUiojzEeuU1cvqHw4MjQ,
      authority:xnBZuojzEeurQLsIowkyDw .
  
  sub:sig npx:hasAlgorithm "RSA";
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB";
    npx:hasSignature "W9laKw4bq47RsI1OPOei9KJEm7F9q05k/+/1xYXLS4aNfpXCHLnH/x/FEDF2tcrw6xPyA8cb+RgIpjcg0pgTBIjwcIJjjFqbknVu0E+RpcElDLv5SlCNzeFb45trEy45T1H6yEWrnbwOudjnMsjLBEAho5mfYVFn+0y/yQbM7lc=";
    npx:hasSignatureTarget this: .
  
  this: npx:introduces sub:step;
    prov:generatedAtTime "2021-03-20T05:39:07.806327"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/iannotate> .
}