Nanopublications

@prefix pplan: <http://purl.org/net/p-plan#> .
@prefix this: <http://purl.org/np/RAxOyWAchk4vIMZsStGUHMVVWOxtdylGRSBZ2oP4kSyZo> .
@prefix sub: <http://purl.org/np/RAxOyWAchk4vIMZsStGUHMVVWOxtdylGRSBZ2oP4kSyZo#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix dc: <http://purl.org/dc/elements/1.1/> .

sub:Head {
  this: np:hasAssertion sub:assertion ;
    np:hasProvenance sub:provenance ;
    np:hasPublicationInfo sub:pubInfo ;
    a np:Nanopublication .
}

sub:assertion {
  <http://purl.obolibrary.org/obo/CHEBI_8984> rdfs:label "10% SDS solution" .
  <http://purl.obolibrary.org/obo/OBI_0400155> rdfs:label "water bath" .
  sub:_1 rdfs:label "Proteinase K solution" .
  sub:step dc:description "Add 1 ml of 10% SDS solution (final concentration, ~1%) and 20 µl of 10 mg/ml Proteinase K solution (final concentration 20 µg/ml). Incubate for 2 to 4 h at 60 °C in a hot water bath." ;
    a pplan:Step ;
    bs:labEquipmentUsed <http://purl.obolibrary.org/obo/OBI_0400155> ;
    bs:reagentUsed <http://purl.obolibrary.org/obo/CHEBI_8984> , sub:_1 .
}

sub:provenance {
  sub:assertion pav:createdWith <https://nanotate.bitsfetch.com> ;
    prov:generatedAtTime "2021-02-26T09:51:16.417108"^^xsd:dateTime ;
    prov:wasAttributedTo <https://hypothes.is/users/zuluaga.lizzz> .
}

sub:pubInfo {
  sub:assertion prov:wasDerivedFrom authority:Qroi1Hg9EeuHDHeRZIV_Ig , authority:nG1-qHg8EeuTh9uL82vmBg , authority:r1bhlHg8EeuhsC8baUEuXw , authority:yoPrang8EeuISTuXTVehFQ .
  sub:sig npx:hasAlgorithm "RSA" ;
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB" ;
    npx:hasSignature "IY+tUv7Gh3sCivIO2+gTbbs1+NNKqsBKBRiQUfNsUrq5f4cQIvfJrSAxOeVivcq29Bzx+YSIfaLy+hZo58F1wLwoggiwj3H6UUtIC8NO8zyYpN6uEf0OVJT4ttH9TrottHWn0NoKVMfWlnxNNzAH6rxGqu9DUAecOkbXmjeDp3U=" ;
    npx:hasSignatureTarget this: .
  this: npx:introduces sub:step ;
    prov:generatedAtTime "2021-02-26T09:51:16.417108"^^xsd:dateTime ;
    prov:wasAttributedTo <https://hypothes.is/users/zuluaga.lizzz> .
}