@prefix pplan: <http://purl.org/net/p-plan#> .
@prefix this: <http://purl.org/np/RAxnrQI8f4DIPUX9dWhFhQVZcNDSvAqMneH92SHF36s88> .
@prefix sub: <http://purl.org/np/RAxnrQI8f4DIPUX9dWhFhQVZcNDSvAqMneH92SHF36s88#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix sp: <http://purl.org/net/SMARTprotocol#> .
@prefix dc: <http://purl.org/dc/elements/1.1/> .
sub:Head {
  this: np:hasAssertion sub:assertion ;
    np:hasProvenance sub:provenance ;
    np:hasPublicationInfo sub:pubInfo ;
    a np:Nanopublication .
}
sub:assertion {
  <http://purl.obolibrary.org/obo/CHEBI_16236> rdfs:label "70% EtOH." .
  <http://purl.obolibrary.org/obo/CHEBI_33696> rdfs:label " nucleic acid" .
  sub:step dc:description "Discard the supernatant and wash the precipitate nucleic acid gently with 70% EtOH." ;
    sp:hasExperimentalOutput <http://purl.obolibrary.org/obo/CHEBI_33696> ;
    a pplan:Step ;
    bs:bioSampleUsed <http://purl.obolibrary.org/obo/CHEBI_33696> ;
    bs:reagentUsed <http://purl.obolibrary.org/obo/CHEBI_16236> .
}
sub:provenance {
  sub:assertion pav:createdWith <https://nanotate.bitsfetch.com> ;
    prov:generatedAtTime "2021-03-05T11:37:08.443992"^^xsd:dateTime ;
    prov:wasAttributedTo <https://hypothes.is/users/iannotate> .
}
sub:pubInfo {
  sub:assertion prov:wasDerivedFrom authority:PxMe7H3JEeupEhdh7oM6aA , authority:WcBt5H3JEeuhAQ_rMFwvBg , authority:cKl68H3JEeuT_PuT2HXdBQ .
  sub:sig npx:hasAlgorithm "RSA" ;
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB" ;
    npx:hasSignature "UMvwOuXeZUmujgSz1QINQHyMiJQ/CnIy5SLm7DlIdfu5iQA4vV/amUCP96Kc0Gf9KXra73pnM6zrpUaBwHhndl371BbqjiKsSaaACIEKHiti1le0KJJ/hJ1b/qzubqI/1xbn/o60GG+ZgUyvcsIrooa0ZKiGqHjvcIRXp6934eA=" ;
    npx:hasSignatureTarget this: .
  this: npx:introduces sub:step ;
    prov:generatedAtTime "2021-03-05T11:37:08.443992"^^xsd:dateTime ;
    prov:wasAttributedTo <https://hypothes.is/users/iannotate> .
}