sub:assertion {
  <http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C62218> rdfs:label "70% ethanol" .
  sub:_1 rdfs:label "DNA " .
  sub:_2 rdfs:label "DNA " .
  sub:_3 rdfs:label "pellet" .
  sub:_4 rdfs:label "tubes" .
  sub:_5 rdfs:label "TE" .
  sub:step dc:description "After incubation, the tubes were centrifuged at 5000 × g for 10 min at 4°C and the supernatant was gently removed. The pellet is washed two times with 1ml of 70% ethanol and the DNA is pellet by 5000 × g at 4°C for only 5 min. The supernatant is discarded and the pellet is air-dried (10 min). The pellet are allowed to re-suspend in 50 μL of TE (10 mM Tris. HCl pH 8.0; 1 mM EDTA pH 8.0)." ;
    sp:hasExperimentalOutput sub:_1 ;
    a pplan:Step ;
    bs:bioSampleUsed sub:_2 , sub:_3 ;
    bs:labEquipmentUsed sub:_4 ;
    bs:reagentUsed <http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C62218> , sub:_5 .
}