@prefix pplan: <http://purl.org/net/p-plan#> .
@prefix this: <http://purl.org/np/RA0gawsiQie0bcSticoo7Iu48RZ6VebPCFzPvfCYDaggA> .
@prefix sub: <http://purl.org/np/RA0gawsiQie0bcSticoo7Iu48RZ6VebPCFzPvfCYDaggA#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix dc: <http://purl.org/dc/elements/1.1/> .
sub:Head {
   this: np:hasAssertion sub:assertion ;
    np:hasProvenance sub:provenance ;
    np:hasPublicationInfo sub:pubInfo ;
    a np:Nanopublication .
}
sub:assertion {
   sub:step dc:description "Centrifuge at 12,000g for 30 minutes at 4º celcius and then  remove the supernatant ." ;
    a pplan:Step .
}
sub:provenance {
   sub:assertion pav:createdWith <https://nanotate.bitsfetch.com> ;
    prov:generatedAtTime "2021-03-11T10:49:02.879315"^^xsd:dateTime ;
    prov:wasAttributedTo <https://hypothes.is/users/iannotate> .
}
sub:pubInfo {
   sub:assertion prov:wasDerivedFrom authority:5hOePIJ_Eeue5Bd7NOrEBA .
  sub:sig npx:hasAlgorithm "RSA" ;
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB" ;
    npx:hasSignature "Fi0+TIdQFAVtWRi9jDK+JBnOTRXQTwtvRJ82pHgVYeysEcbqBYeRWuwX+y3ktHH1XcAdPBJ8StsiUXQ6h8UcwYOB/t9Sco8Q5GjImp/R+6Hz6tgXola6RWuufKYzq20rv/9IGu8b4lLVSuxxnAhig38o9CGpwbJeHQgMomXXG5E=" ;
    npx:hasSignatureTarget this: .
  this: npx:introduces sub:step ;
    prov:generatedAtTime "2021-03-11T10:49:02.879315"^^xsd:dateTime ;
    prov:wasAttributedTo <https://hypothes.is/users/iannotate> .
}