@prefix pplan: <http://purl.org/net/p-plan#> .
@prefix this: <http://purl.org/np/RA4i76gW52Hs0wwMZGJavBEJnPC-4huZHXTvu6zftDauI> .
@prefix sub: <http://purl.org/np/RA4i76gW52Hs0wwMZGJavBEJnPC-4huZHXTvu6zftDauI#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix sp: <http://purl.org/net/SMARTprotocol#> .
@prefix dc: <http://purl.org/dc/elements/1.1/> .
sub:Head {
   this: np:hasAssertion sub:assertion ;
    np:hasProvenance sub:provenance ;
    np:hasPublicationInfo sub:pubInfo ;
    a np:Nanopublication .
}
sub:assertion {
   <http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C62218> rdfs:label "70% ethanol" .
  <http://purl.obolibrary.org/obo/UBERON_0036018> rdfs:label "pellet" .
  sub:_1 rdfs:label "DNA " .
  sub:_2 rdfs:label "DNA " .
  sub:_3 rdfs:label "TE" .
  sub:_4 rdfs:label "tubes" .
  sub:step dc:description "After incubation, the tubes were centrifuged at 5000 × g for 10 min at 4°C and the supernatant was gently removed. The pellet is washed two times with 1ml of 70% ethanol and the DNA is pellet by 5000 × g at 4°C for only 5 min. The supernatant is discarded and the pellet is air-dried (10 min). The pellet are allowed to re-suspend in 50 μL of TE (10 mM Tris. HCl pH 8.0; 1 mM EDTA pH 8.0)." ;
    sp:hasExperimentalOutput sub:_1 ;
    a pplan:Step ;
    bs:bioSampleUsed <http://purl.obolibrary.org/obo/UBERON_0036018> , sub:_2 ;
    bs:reagentUsed <http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C62218> , sub:_3 , sub:_4 .
}
sub:provenance {
   sub:assertion pav:createdWith <https://nanotate.bitsfetch.com> ;
    prov:generatedAtTime "2021-03-10T10:48:12.163485"^^xsd:dateTime ;
    prov:wasAttributedTo <https://hypothes.is/users/alejandra.sandoval> .
}
sub:pubInfo {
   sub:assertion prov:wasDerivedFrom authority:LCpvRoFOEeuIKp8RxBt8IQ , authority:UwzjpoFNEeuO5vPfHtOEXw , authority:_7_TpoFNEeu3wv9aWnG4oQ , authority:fRxWbIFOEeu3xVt13uxEUw , authority:iIepxoFNEeukrhvbYqQvHQ , authority:n1MtvoFOEeua_Nfa0WTqLA .
  sub:sig npx:hasAlgorithm "RSA" ;
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB" ;
    npx:hasSignature "RMaOJcdPWFjAA8j9YXQcOybXfN55KO5NkbW0ejJC/rED9NtOPEQyGn7HaA2211rSGdL0BVPAlq8abrJbKnTxWBXDDGFHvceyrIhrZbMdM7bDu7pGFHtPmmwdW8cby7aQQQvLVpNrB93Xu7AyagXSkdOl05y3FQsvxlDAgf2USl8=" ;
    npx:hasSignatureTarget this: .
  this: npx:introduces sub:step ;
    prov:generatedAtTime "2021-03-10T10:48:12.163485"^^xsd:dateTime ;
    prov:wasAttributedTo <https://hypothes.is/users/alejandra.sandoval> .
}