@prefix pplan: <
http://purl.org/net/p-plan#
> .
@prefix this: <
http://purl.org/np/RA6P-8z4UVxXMPLzAnfceF9udgMugYt6C_19GiLn-WauE
> .
@prefix sub: <
http://purl.org/np/RA6P-8z4UVxXMPLzAnfceF9udgMugYt6C_19GiLn-WauE#
> .
@prefix bs: <
https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#
> .
@prefix np: <
http://www.nanopub.org/nschema#
> .
@prefix pav: <
http://purl.org/pav/
> .
@prefix authority: <
https://hypothes.is/a/
> .
@prefix npx: <
http://purl.org/nanopub/x/
> .
@prefix xsd: <
http://www.w3.org/2001/XMLSchema#
> .
@prefix rdfs: <
http://www.w3.org/2000/01/rdf-schema#
> .
@prefix prov: <
http://www.w3.org/ns/prov#
> .
@prefix sp: <
http://purl.org/net/SMARTprotocol#
> .
@prefix dc: <
http://purl.org/dc/elements/1.1/
> .
sub:Head
{
this:
np:hasAssertion
sub:assertion
;
np:hasProvenance
sub:provenance
;
np:hasPublicationInfo
sub:pubInfo
;
a
np:Nanopublication
.
}
sub:assertion
{
<
http://purl.obolibrary.org/obo/OBI_0002488
>
rdfs:label
"pipette" .
sub:_1
rdfs:label
"mycobacteria" .
sub:_2
rdfs:label
"mycobacteria" .
sub:_3
rdfs:label
"log phase culture" .
sub:_4
rdfs:label
"TE" .
sub:step
dc:description
"""Harvest mycobacteria by centrifuging 20-40 ml log phase culture (O.D.600 ≈ 0.8) at 2,000 x g for 10 min, room temperature. Carefully discard culture medium and add 10 ml TE (at room temperature) buffer to pellet. Mix gently with a pipette.
Note: If isolating DNA from pathogenic mycobacteria such as M. tuberculosis, step 1 and 2 must be performed in a BSL-3 facility.""" ;
sp:hasExperimentalInput
sub:_1
;
a
pplan:Step
;
bs:bioSampleUsed
sub:_2
;
bs:labEquipmentUsed
<
http://purl.obolibrary.org/obo/OBI_0002488
> ;
bs:reagentUsed
sub:_3
,
sub:_4
.
}
sub:provenance
{
sub:assertion
pav:createdWith
<
https://nanotate.bitsfetch.com
> ;
prov:generatedAtTime
"2021-02-26T09:45:59.609387"^^
xsd:dateTime
;
prov:wasAttributedTo
<
https://hypothes.is/users/zuluaga.lizzz
> .
}
sub:pubInfo
{
sub:assertion
prov:wasDerivedFrom
authority:Omq7ZnexEeueAA-4Ziqxeg
,
authority:fpY55nexEeuGACeYmzK6Tw
,
authority:mSDgSnexEeuhY99_AxTSHA
,
authority:q_iw0HexEeu8DDM9AtM47w
,
authority:zKzFyHexEeu14yM5lwg2aA
.
sub:sig
npx:hasAlgorithm
"RSA" ;
npx:hasPublicKey
"MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB" ;
npx:hasSignature
"VuLlQnlwr/b3r9gKLFepvxdP/9l1lLHIQc6DvAmlTsrDuRMiS5ziKhRmRlYZBTXS+aJVCOgEwl1c3Wadno5aVWqGXJs27W46ITDK/shlepXJ6bimA/NRQ/0sjRJqT+F+ctf+dCEEqetRwGLJeO2stB/0AyYRrSUix8Z3QkiItdM=" ;
npx:hasSignatureTarget
this:
.
this:
npx:introduces
sub:step
;
prov:generatedAtTime
"2021-02-26T09:45:59.609387"^^
xsd:dateTime
;
prov:wasAttributedTo
<
https://hypothes.is/users/zuluaga.lizzz
> .
}