@prefix pplan: <http://purl.org/net/p-plan#> .
@prefix this: <http://purl.org/np/RA6U6VQH2TFco9whEYEq4SGF-xhT-qxATbkJ1QFblfOiE> .
@prefix sub: <http://purl.org/np/RA6U6VQH2TFco9whEYEq4SGF-xhT-qxATbkJ1QFblfOiE#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix dc: <http://purl.org/dc/elements/1.1/> .
sub:Head {
   this: np:hasAssertion sub:assertion ;
    np:hasProvenance sub:provenance ;
    np:hasPublicationInfo sub:pubInfo ;
    a np:Nanopublication .
}
sub:assertion {
   sub:_1 rdfs:label "cell suspension" .
  sub:_2 rdfs:label "24 well culture plate" .
  sub:step dc:description """Seed 500 µl of cell suspension in a 24 well culture plate.
Note: Monocytes in PBMCs get attached to the plastic in about 2-3 h when incubated at 37 °C. Longer incubation will result in firm attachement. Lymphocytes are not glass adherent and they will be mostly in suspension and can be removed by mildly flushing the wells with medium and/or buffer. Such treatment will keep the monocytes firmly attached to the surface of culture plates.""" ;
    a pplan:Step ;
    bs:bioSampleUsed sub:_1 ;
    bs:labEquipmentUsed sub:_2 .
}
sub:provenance {
   sub:assertion pav:createdWith <https://nanotate.bitsfetch.com> ;
    prov:generatedAtTime "2021-02-14T05:25:31.936259"^^xsd:dateTime ;
    prov:wasAttributedTo <https://hypothes.is/users/zuluaga.lizzz> .
}
sub:pubInfo {
   sub:assertion prov:wasDerivedFrom authority:V-kpfG6uEeunoAOTPgQJFQ , authority:ba-t2m6uEeucdPvQv1fpyQ , authority:hL_d4m6uEeu2HOeqlNADQQ .
  sub:sig npx:hasAlgorithm "RSA" ;
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB" ;
    npx:hasSignature "Ch1XrzNj8/n2Mc6yIB9Fai8SHUGy3PrCF6yLDxK6pGSXqqzy3euLyc/18I3YRXluHBYYfuLBOXymd4ifAoc400tey7BpGghyjOmzcHB6b1JtMrJhB+9p2di0t33ZUZxdKY24QlWcXLofyFF+zHamD4uByv2I0ZpSVmVYMdurr/w=" ;
    npx:hasSignatureTarget this: .
  this: npx:introduces sub:step ;
    prov:generatedAtTime "2021-02-14T05:25:31.936259"^^xsd:dateTime ;
    prov:wasAttributedTo <https://hypothes.is/users/zuluaga.lizzz> .
}