@prefix pplan: <http://purl.org/net/p-plan#> .
@prefix this: <http://purl.org/np/RA7w23aq6cPWBsg32SVRq31TjCUhyFii5YzHMXu0ArKBs> .
@prefix sub: <http://purl.org/np/RA7w23aq6cPWBsg32SVRq31TjCUhyFii5YzHMXu0ArKBs#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix dc: <http://purl.org/dc/elements/1.1/> .
sub:Head {
   this: np:hasAssertion sub:assertion ;
    np:hasProvenance sub:provenance ;
    np:hasPublicationInfo sub:pubInfo ;
    a np:Nanopublication .
}
sub:assertion {
   sub:_1 rdfs:label "supernatant" .
  sub:_2 rdfs:label "2ml Eppendorf tubes" .
  sub:_3 rdfs:label "chloroform: isoamyl alcohol" .
  sub:step dc:description "Transfer 1ml of supernatant to each 2ml Eppendorf tubes already containing 1 ml of chloroform: isoamyl alcohol (24:1). Mix supernatant and chloroform: isoamyl alcohol by gentle inversions for 10 min and subsequently place the tube on ice for 10 min." ;
    a pplan:Step ;
    bs:bioSampleUsed sub:_1 ;
    bs:labEquipmentUsed sub:_2 ;
    bs:reagentUsed sub:_3 .
}
sub:provenance {
   sub:assertion pav:createdWith <https://nanotate.bitsfetch.com> ;
    prov:generatedAtTime "2021-03-11T16:43:40.490445"^^xsd:dateTime ;
    prov:wasAttributedTo <https://hypothes.is/users/iannotate> .
}
sub:pubInfo {
   sub:assertion prov:wasDerivedFrom authority:3LrRXoKvEeuUAo9ZCa5XBQ , authority:7ztorIKvEeuA0a-v9M3Ymw , authority:BzJ0KIKwEeuzuleay-H5tw , authority:zheK1IKvEeufaDPs6pTx5w .
  sub:sig npx:hasAlgorithm "RSA" ;
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB" ;
    npx:hasSignature "UBe9joOiP7DcioVtQ/gVeaN8yAJVIvUhTmYo4D1ybqbRBucZTvgJKUrYb6IHa2vVWn6rnJFs1m9LnSP5hgrXQUIEQjj1metAY64OG9M9MxpeMAzha32XqzutZMndxYqSCkr3k5mILe4be6OpbrPCYlURSB4LIBxARhiizjV3fQw=" ;
    npx:hasSignatureTarget this: .
  this: npx:introduces sub:step ;
    prov:generatedAtTime "2021-03-11T16:43:40.490445"^^xsd:dateTime ;
    prov:wasAttributedTo <https://hypothes.is/users/iannotate> .
}