@prefix pplan: <
http://purl.org/net/p-plan#
> .
@prefix this: <
http://purl.org/np/RA9UL01PNcDPZrtyBTTJgTpV6h6qOdw1CJrUY5lAge7FE
> .
@prefix sub: <
http://purl.org/np/RA9UL01PNcDPZrtyBTTJgTpV6h6qOdw1CJrUY5lAge7FE#
> .
@prefix bs: <
https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#
> .
@prefix np: <
http://www.nanopub.org/nschema#
> .
@prefix pav: <
http://purl.org/pav/
> .
@prefix authority: <
https://hypothes.is/a/
> .
@prefix npx: <
http://purl.org/nanopub/x/
> .
@prefix xsd: <
http://www.w3.org/2001/XMLSchema#
> .
@prefix rdfs: <
http://www.w3.org/2000/01/rdf-schema#
> .
@prefix prov: <
http://www.w3.org/ns/prov#
> .
@prefix sp: <
http://purl.org/net/SMARTprotocol#
> .
@prefix dc: <
http://purl.org/dc/elements/1.1/
> .
sub:Head
{
this:
np:hasAssertion
sub:assertion
;
np:hasProvenance
sub:provenance
;
np:hasPublicationInfo
sub:pubInfo
;
a
np:Nanopublication
.
}
sub:assertion
{
<
http://purl.obolibrary.org/obo/CHEBI_16991
>
rdfs:label
"DNA " .
sub:_1
rdfs:label
"pellet" .
sub:_2
rdfs:label
"nuclease free water" .
sub:_3
rdfs:label
"TE buffer" .
sub:step
dc:description
"Dissolve the dry pellet in nuclease free water or TE buffer (Ph 8.0). Store DNA at 4 º celcius or -20 º celcius" ;
sp:hasExperimentalOutput
<
http://purl.obolibrary.org/obo/CHEBI_16991
> ;
a
pplan:Step
;
bs:bioSampleUsed
<
http://purl.obolibrary.org/obo/CHEBI_16991
> ,
sub:_1
;
bs:reagentUsed
sub:_2
,
sub:_3
.
}
sub:provenance
{
sub:assertion
pav:createdWith
<
https://nanotate.bitsfetch.com
> ;
prov:generatedAtTime
"2021-03-10T10:30:30.319889"^^
xsd:dateTime
;
prov:wasAttributedTo
<
https://hypothes.is/users/alejandra.sandoval
> .
}
sub:pubInfo
{
sub:assertion
prov:wasDerivedFrom
authority:BsLfvnJSEeuwwHON4jxSyA
,
authority:OB9gTIG1Eeumnrv5E-jW0A
,
authority:R0LJMnJSEeu0aSvlE0AT3g
,
authority:dEsg9oG1Eeu2Dd_XOCmlpg
,
authority:z-YCRnJREeuh6BcZAJ90mg
.
sub:sig
npx:hasAlgorithm
"RSA" ;
npx:hasPublicKey
"MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB" ;
npx:hasSignature
"G5Y/0rB3G4IIC/MXqrSHVVTqcrwqnk5AySRkadKh1JjthSUKYPshsspEwJY6VQ8o3sV6fUN4tlaWVPTsPpN7d2KfqLOqqaFNDz3nI47rqCnIVNlStBjcVjMvXlD1N8V9S9lUXFd4zAnnjaiZNjn2bITDSH6ayFgyUFfZj47Y/u8=" ;
npx:hasSignatureTarget
this:
.
this:
npx:introduces
sub:step
;
prov:generatedAtTime
"2021-03-10T10:30:30.319889"^^
xsd:dateTime
;
prov:wasAttributedTo
<
https://hypothes.is/users/alejandra.sandoval
> .
}