@prefix pplan: <http://purl.org/net/p-plan#> .
@prefix this: <http://purl.org/np/RA9UL01PNcDPZrtyBTTJgTpV6h6qOdw1CJrUY5lAge7FE> .
@prefix sub: <http://purl.org/np/RA9UL01PNcDPZrtyBTTJgTpV6h6qOdw1CJrUY5lAge7FE#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix sp: <http://purl.org/net/SMARTprotocol#> .
@prefix dc: <http://purl.org/dc/elements/1.1/> .
sub:Head {
  this: np:hasAssertion sub:assertion ;
    np:hasProvenance sub:provenance ;
    np:hasPublicationInfo sub:pubInfo ;
    a np:Nanopublication .
}
sub:assertion {
  <http://purl.obolibrary.org/obo/CHEBI_16991> rdfs:label "DNA " .
  sub:_1 rdfs:label "pellet" .
  sub:_2 rdfs:label "nuclease free water" .
  sub:_3 rdfs:label "TE buffer" .
  sub:step dc:description "Dissolve the dry pellet in nuclease free water or TE buffer (Ph 8.0). Store DNA at 4 º celcius  or -20 º celcius" ;
    sp:hasExperimentalOutput <http://purl.obolibrary.org/obo/CHEBI_16991> ;
    a pplan:Step ;
    bs:bioSampleUsed <http://purl.obolibrary.org/obo/CHEBI_16991> , sub:_1 ;
    bs:reagentUsed sub:_2 , sub:_3 .
}
sub:provenance {
  sub:assertion pav:createdWith <https://nanotate.bitsfetch.com> ;
    prov:generatedAtTime "2021-03-10T10:30:30.319889"^^xsd:dateTime ;
    prov:wasAttributedTo <https://hypothes.is/users/alejandra.sandoval> .
}
sub:pubInfo {
  sub:assertion prov:wasDerivedFrom authority:BsLfvnJSEeuwwHON4jxSyA , authority:OB9gTIG1Eeumnrv5E-jW0A , authority:R0LJMnJSEeu0aSvlE0AT3g , authority:dEsg9oG1Eeu2Dd_XOCmlpg , authority:z-YCRnJREeuh6BcZAJ90mg .
  sub:sig npx:hasAlgorithm "RSA" ;
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB" ;
    npx:hasSignature "G5Y/0rB3G4IIC/MXqrSHVVTqcrwqnk5AySRkadKh1JjthSUKYPshsspEwJY6VQ8o3sV6fUN4tlaWVPTsPpN7d2KfqLOqqaFNDz3nI47rqCnIVNlStBjcVjMvXlD1N8V9S9lUXFd4zAnnjaiZNjn2bITDSH6ayFgyUFfZj47Y/u8=" ;
    npx:hasSignatureTarget this: .
  this: npx:introduces sub:step ;
    prov:generatedAtTime "2021-03-10T10:30:30.319889"^^xsd:dateTime ;
    prov:wasAttributedTo <https://hypothes.is/users/alejandra.sandoval> .
}