@prefix obo: <http://purl.obolibrary.org/obo/> .
@prefix dce: <http://purl.org/dc/elements/1.1/> .
@prefix pplan: <http://purl.org/net/p-plan#> .
@prefix this: <http://purl.org/np/RAAVxMa4yCOeoI-9JCEoT2UY2EzTbR8SF4fWWFSY_vAT8> .
@prefix sub: <http://purl.org/np/RAAVxMa4yCOeoI-9JCEoT2UY2EzTbR8SF4fWWFSY_vAT8#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
sub:Head {
   this: np:hasAssertion sub:assertion ;
    np:hasProvenance sub:provenance ;
    np:hasPublicationInfo sub:pubInfo ;
    a np:Nanopublication .
}
sub:assertion {
   obo:CHEBI_16236 rdfs:label "95% ethanol" .
  obo:ERO_0000979 rdfs:label "freezer" .
  sub:_1 rdfs:label "tubes" .
  sub:step dce:description "Add 2 volumes of cold 95% ethanol and mix gently by inversion. The tubes are incubated at for 45 min in -20°C freezer. It should not be for more time as some remaining phenolics and resin may also precipitate with DNA." ;
    a pplan:Step ;
    bs:labEquipmentUsed obo:ERO_0000979 , sub:_1 ;
    bs:reagentUsed obo:CHEBI_16236 .
}
sub:provenance {
   sub:assertion pav:createdWith <https://nanotate.bitsfetch.com> ;
    prov:generatedAtTime "2021-03-11T16:41:02.775153"^^xsd:dateTime ;
    prov:wasAttributedTo <https://hypothes.is/users/iannotate> .
}
sub:pubInfo {
   sub:assertion prov:wasDerivedFrom authority:XpTlLoKxEeunl4Np8U_8-w , authority:dA4nJoKxEeuZYgOgDpIN0w , authority:h3tp6oKxEeuj8luk3y1vPA , authority:oEVJloKxEeu8zcs9BHRJgA .
  sub:sig npx:hasAlgorithm "RSA" ;
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB" ;
    npx:hasSignature "Dm+vcYy8uU6NXFhEeKWTr37B/P6+9m8UxKrzQidhYNJv4ALVJup0f98tg+gw0f/Y97UjuvaMxpy8j6K7TrPS9Yj0CkWeqb0u0Xb5+CayKpn0ni3/9FEAzRiMHa5I/L1mfI9xp/9ZVX6X5CIJ7HMHm2QaGzpUMAnkle2DStuo7Gk=" ;
    npx:hasSignatureTarget this: .
  this: npx:introduces sub:step ;
    prov:generatedAtTime "2021-03-11T16:41:02.775153"^^xsd:dateTime ;
    prov:wasAttributedTo <https://hypothes.is/users/iannotate> .
}