@prefix obo: <
http://purl.obolibrary.org/obo/
> .
@prefix dce: <
http://purl.org/dc/elements/1.1/
> .
@prefix pplan: <
http://purl.org/net/p-plan#
> .
@prefix this: <
http://purl.org/np/RAAb0oBQSjGFE8J9e7QQ64pIzSw6Zf-wghGZap0bk3Pyg
> .
@prefix sub: <
http://purl.org/np/RAAb0oBQSjGFE8J9e7QQ64pIzSw6Zf-wghGZap0bk3Pyg#
> .
@prefix bs: <
https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#
> .
@prefix np: <
http://www.nanopub.org/nschema#
> .
@prefix pav: <
http://purl.org/pav/
> .
@prefix authority: <
https://hypothes.is/a/
> .
@prefix npx: <
http://purl.org/nanopub/x/
> .
@prefix xsd: <
http://www.w3.org/2001/XMLSchema#
> .
@prefix rdfs: <
http://www.w3.org/2000/01/rdf-schema#
> .
@prefix prov: <
http://www.w3.org/ns/prov#
> .
@prefix sp: <
http://purl.org/net/SMARTprotocol#
> .
sub:Head
{
this:
np:hasAssertion
sub:assertion
;
np:hasProvenance
sub:provenance
;
np:hasPublicationInfo
sub:pubInfo
;
a
np:Nanopublication
.
}
sub:assertion
{
obo:CHEBI_16991
rdfs:label
"DNA" .
sp:SeventyFivePercentEthanol
rdfs:label
"75% ethanol." .
sub:step
dce:description
"Wash DNA pellet by adding 10 ml of 75% ethanol. Centrifuge at 12,000 x g for 10 min, 4 °C." ;
sp:hasExperimentalOutput
obo:CHEBI_16991
;
a
pplan:Step
;
bs:bioSampleUsed
obo:CHEBI_16991
;
bs:reagentUsed
sp:SeventyFivePercentEthanol
.
}
sub:provenance
{
sub:assertion
pav:createdWith
<
https://nanotate.bitsfetch.com
> ;
prov:generatedAtTime
"2021-02-26T09:48:23.647434"^^
xsd:dateTime
;
prov:wasAttributedTo
<
https://hypothes.is/users/zuluaga.lizzz
> .
}
sub:pubInfo
{
sub:assertion
prov:wasDerivedFrom
authority:1W95jng-EeupiidDEq_igg
,
authority:rE6wTHg-EeubtM9pye88Nw
,
authority:wPdFBHg-EeuUE88CGquTDg
.
sub:sig
npx:hasAlgorithm
"RSA" ;
npx:hasPublicKey
"MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB" ;
npx:hasSignature
"WKwJTnsFWqiS87bQEtLHaya3UG3Y8wo5U484mWbhl5BY6W1ulAu41X7eFaLqrm7I6zFgkBKKdNyJfR3tjOfc9w1Id91G1thzsh36v+2dT+FeZ3c/Wy29caQ0+b7S9MidH6VYcO6sqOdIuoFG9eF+6MkjwPd3fM2W1PE6Mr0Gzms=" ;
npx:hasSignatureTarget
this:
.
this:
npx:introduces
sub:step
;
prov:generatedAtTime
"2021-02-26T09:48:23.647434"^^
xsd:dateTime
;
prov:wasAttributedTo
<
https://hypothes.is/users/zuluaga.lizzz
> .
}