@prefix dce: <http://purl.org/dc/elements/1.1/> .
@prefix pplan: <http://purl.org/net/p-plan#> .
@prefix this: <http://purl.org/np/RAAsE85KG2Q2ha1boFSh1aJW3m1Qt3zQH_BePeybp3-hs> .
@prefix sub: <http://purl.org/np/RAAsE85KG2Q2ha1boFSh1aJW3m1Qt3zQH_BePeybp3-hs#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
sub:Head {
   this: np:hasAssertion sub:assertion ;
    np:hasProvenance sub:provenance ;
    np:hasPublicationInfo sub:pubInfo ;
    a np:Nanopublication .
}
sub:assertion {
   sub:_1 rdfs:label "pellet." .
  sub:step dce:description "After centrifuging at 5400 g at 4˚C for 5 min, remove the supernatant and then air-dry the pellet." ;
    a pplan:Step ;
    bs:bioSampleUsed sub:_1 .
}
sub:provenance {
   sub:assertion pav:createdWith <https://nanotate.bitsfetch.com> ;
    prov:generatedAtTime "2021-03-05T11:36:37.574643"^^xsd:dateTime ;
    prov:wasAttributedTo <https://hypothes.is/users/iannotate> .
}
sub:pubInfo {
   sub:assertion prov:wasDerivedFrom authority:gU8kpH3JEeuOU7eochtG0w , authority:oNg5ln3JEeute888DPv69A .
  sub:sig npx:hasAlgorithm "RSA" ;
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB" ;
    npx:hasSignature "NqEl0ouVMJFnVtOL30gnRRee7S7T4Z54z7rXOaN8EY+CSuFLDNXdjhLK7XvQ6ALDjJwGgEVnp9MUcST4rVneTeGHrW524pkYcPGVTMTN4ST3WsCHh8ECLBIS3JHCCSFghNXtqBDRYfca/HC0gaZI0CA1oBFS4Wlyay9SXB1oyjE=" ;
    npx:hasSignatureTarget this: .
  this: npx:introduces sub:step ;
    prov:generatedAtTime "2021-03-05T11:36:37.574643"^^xsd:dateTime ;
    prov:wasAttributedTo <https://hypothes.is/users/iannotate> .
}