@prefix obo: <
http://purl.obolibrary.org/obo/
> .
@prefix dce: <
http://purl.org/dc/elements/1.1/
> .
@prefix pplan: <
http://purl.org/net/p-plan#
> .
@prefix this: <
http://purl.org/np/RAE99H9MwYVHT12e7hbsUsBBLdAuQv0jr7JInE5tFiPs4
> .
@prefix sub: <
http://purl.org/np/RAE99H9MwYVHT12e7hbsUsBBLdAuQv0jr7JInE5tFiPs4#
> .
@prefix bs: <
https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#
> .
@prefix np: <
http://www.nanopub.org/nschema#
> .
@prefix pav: <
http://purl.org/pav/
> .
@prefix authority: <
https://hypothes.is/a/
> .
@prefix npx: <
http://purl.org/nanopub/x/
> .
@prefix xsd: <
http://www.w3.org/2001/XMLSchema#
> .
@prefix rdfs: <
http://www.w3.org/2000/01/rdf-schema#
> .
@prefix prov: <
http://www.w3.org/ns/prov#
> .
@prefix sp: <
http://purl.org/net/SMARTprotocol#
> .
sub:Head
{
this:
np:hasAssertion
sub:assertion
;
np:hasProvenance
sub:provenance
;
np:hasPublicationInfo
sub:pubInfo
;
a
np:Nanopublication
.
}
sub:assertion
{
obo:CHEBI_35255
rdfs:label
"chloroform" .
sub:_1
rdfs:label
"Cell lysates " .
sub:_2
rdfs:label
"Cell lysates " .
sub:_3
rdfs:label
"eppendorf tubes" .
sub:step
dce:description
"Cell lysates were transfer into eppendorf tubes and one-fifth (volume/volume) of chloroform was added to each tube." ;
sp:hasExperimentalInput
sub:_1
;
a
pplan:Step
;
bs:bioSampleUsed
sub:_2
;
bs:labEquipmentUsed
sub:_3
;
bs:reagentUsed
obo:CHEBI_35255
.
}
sub:provenance
{
sub:assertion
pav:createdWith
<
https://nanotate.bitsfetch.com
> ;
prov:generatedAtTime
"2021-02-18T09:48:11.811790"^^
xsd:dateTime
;
prov:wasAttributedTo
<
https://hypothes.is/users/alejandra.sandoval
> .
}
sub:pubInfo
{
sub:assertion
prov:wasDerivedFrom
authority:7_X7wnE-Eeuw3kNBSTg_-A
,
authority:CbGn5nH4Eeuv6guNO1r8uA
,
authority:EbCxOnE_Eeur9ytFzTfK7w
,
authority:Om7khHE_EeunexslNNPfLA
.
sub:sig
npx:hasAlgorithm
"RSA" ;
npx:hasPublicKey
"MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB" ;
npx:hasSignature
"CdzFbuup4Q9hPPF0ngwnWt8W8OAQektRSzEYZPMPrpwL9KAp93ZkX4J8jd+OFEJQSvTutWN0G4q8ySfad5c1jseLuC9iy/fSWQZN4qMDnub58DAJ13poJpuv+fklNnRdzng48n7Jjfg4NhtmmsmyWDn7xH8mwor2t47UyE+ttwA=" ;
npx:hasSignatureTarget
this:
.
this:
npx:introduces
sub:step
;
prov:generatedAtTime
"2021-02-18T09:48:11.811790"^^
xsd:dateTime
;
prov:wasAttributedTo
<
https://hypothes.is/users/alejandra.sandoval
> .
}