sub:assertion {
  obo:OBI_0000918 rdfs:label "Dulbecco's modified eagle medium" .
  sub:_1 rdfs:label "cells" .
  sub:_2 rdfs:label "haemocytometer" .
  sub:_3 rdfs:label "10% FBS" .
  sub:_4 rdfs:label "W.B.C. diluting fluid" .
  sub:_5 rdfs:label "1% of Pencillin-streptomycin solution" .
  sub:step dce:description "Count cells by haemocytometer using W.B.C. diluting fluid: Add 10 µl of cell suspension to 190 µl of W.B.C. diluting fluid and mix well. Load the cell suspension in a haemocytometer and count the cells. Adjust cell concentration at 1 x 106 cells/ml with Dulbecco's modified eagle medium supplemented with 1% of Pencillin-streptomycin solution and 10% FBS. The approximate yield of cells from 4 ml of blood varies between 107-108." ;
    a pplan:Step ;
    bs:bioSampleUsed sub:_1 ;
    bs:labEquipmentUsed sub:_2 ;
    bs:reagentUsed obo:OBI_0000918 , sub:_3 , sub:_4 , sub:_5 .
}