@prefix dce: <http://purl.org/dc/elements/1.1/> .
@prefix pplan: <http://purl.org/net/p-plan#> .
@prefix this: <http://purl.org/np/RAFe_u-PVanc0B0tON93iFuSTZY4eBtkGm6T6rd7yU6nY> .
@prefix sub: <http://purl.org/np/RAFe_u-PVanc0B0tON93iFuSTZY4eBtkGm6T6rd7yU6nY#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
sub:Head {
   this: np:hasAssertion sub:assertion ;
    np:hasProvenance sub:provenance ;
    np:hasPublicationInfo sub:pubInfo ;
    a np:Nanopublication .
}
sub:assertion {
   sub:step dce:description "Centrifuge at 12,000g for 30 minutes at 4º celcius and then  remove the supernatant ." ;
    a pplan:Step .
}
sub:provenance {
   sub:assertion pav:createdWith <https://nanotate.bitsfetch.com> ;
    prov:generatedAtTime "2021-02-24T15:59:40.030721"^^xsd:dateTime ;
    prov:wasAttributedTo <https://hypothes.is/users/zuluaga.lizzz> .
}
sub:pubInfo {
   sub:assertion prov:wasDerivedFrom authority:rYzS-HbhEeueAkuxbkNyrw .
  sub:sig npx:hasAlgorithm "RSA" ;
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB" ;
    npx:hasSignature "EkCRe3moEcET/bqY7HaJQEQBjspZMLBzg6G7iXyfeSbOy7LCInSA+HxnuE8/yHPoni8WHdyn/u3oEw9Khjk3gNa3jF+xwnJp4wbvNKNA0uqBj1WdQiLcajXZxgs6btQEmAFGhCQqR/xkdiOHQPz/MYqmCkt74eieU41UCSS/A/s=" ;
    npx:hasSignatureTarget this: .
  this: npx:introduces sub:step ;
    prov:generatedAtTime "2021-02-24T15:59:40.030721"^^xsd:dateTime ;
    prov:wasAttributedTo <https://hypothes.is/users/zuluaga.lizzz> .
}