@prefix obo: <http://purl.obolibrary.org/obo/> .
@prefix dce: <http://purl.org/dc/elements/1.1/> .
@prefix this: <https://protocolexchange.researchsquare.com/article/pex-1069/v1#RAFt6stRZSIiuA-w5go4rzFRQJci2Ez9yYnRw60sQMcYQ> .
@prefix sub: <https://protocolexchange.researchsquare.com/article/pex-1069/v1#RAFt6stRZSIiuA-w5go4rzFRQJci2Ez9yYnRw60sQMcYQ.> .
@prefix authority: <https://hypothes.is/a/> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix npx: <http://purl.org/nanopub/x/> .
sub:Head {
   this: np:hasAssertion sub:assertion ;
    np:hasProvenance sub:provenance ;
    np:hasPublicationInfo sub:pubInfo ;
    a np:Nanopublication .
  sub:assertion rdfs:member sub:step .
}
sub:assertion {
   obo:CHEBI_18153 rdfs:label "ethylene carbonate" .
  obo:CHEBI_29362 rdfs:label "ethylene carbonate" .
  obo:CHEBI_41609 rdfs:label "ethylene carbonate" .
  sub:step dce:description "DNA point accumulation for imaging in nanoscale topography (DNA-PAINT) facilitates multiplexing in superresolution microscopy but is practically limited by slow imaging speed. We have developed DNA-PAINT-ERS, where E=ethylene carbonate, R=repeating sequence, and S=spacer, for fast and multiplexed superresolution imaging with DNA-PAINT. Here we describe detailed procedures for DNA-PAINT-ERS including reagent preparation, sample labeling, as well as image acqusition and analysis." ;
    bs:bioSampleUsed "microscopy" , obo:CHEBI_18153 , obo:CHEBI_29362 , obo:CHEBI_41609 ;
    bs:reagentUsed obo:CHEBI_18153 , obo:CHEBI_29362 , obo:CHEBI_41609 .
}
sub:provenance {
   sub:assertion prov:generatedAtTime "2020-09-26T04:25:15.310171+00:00"^^xsd:dateTime ;
    prov:wasDerivedFrom authority:Rq3gcP-wEeqPmLtbnUDy2A , authority:icECav-xEeqGzeOtUkOIRw , authority:ok_85v-xEeqsfZ9V8yVNxg .
}
sub:pubInfo {
   this: prov:generatedAtTime "2020-09-26T04:25:15.310171+00:00"^^xsd:dateTime ;
    prov:wasAttributedTo <https://hypothes.is/a/acct:miguel.ruano@hypothes.is> .
  sub:sig npx:hasAlgorithm "RSA" ;
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCAYBVNwcjF5IK+z2KRM4MqV5gG0zVrjoqZtICaimdnS2jGy8YCweNDHUD6ZWfv5p4dWQal1NAMUm1GRXJGwGYIi3ug/zL5YLU72QeDa1ziLjWa2UHHhdGzjoZy+TB87Zhe8LI4fJUXhU+jMwxCG2O613mXmmOpNQBGFJL6+VOyWwIDAQAB" ;
    npx:hasSignature "CZXKmRwXowx9wotw1ylvUjAag63LsoYKjW558pNwKyV1LewFNR9kjaj4fkf06XgumSJwD2gsWU900LHZ9JVT3EBKJaMPvJ5IUBHGEXsE/vRLEDD4+XEDvsQIDQ/j70ql9FRpTtLatEkCeITiVIrPtkP6KDJozMHY/reAVR+dLgo=" ;
    npx:hasSignatureTarget this: .
}