@prefix obo: <http://purl.obolibrary.org/obo/> .
@prefix dce: <http://purl.org/dc/elements/1.1/> .
@prefix pplan: <http://purl.org/net/p-plan#> .
@prefix this: <http://purl.org/np/RAGVyz0_FLr0Oz6EfGaRgsRbnDJMRqvjWGI72AtFO-jhk> .
@prefix sub: <http://purl.org/np/RAGVyz0_FLr0Oz6EfGaRgsRbnDJMRqvjWGI72AtFO-jhk#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix sp: <http://purl.org/net/SMARTprotocol#> .
sub:Head {
  this: np:hasAssertion sub:assertion ;
    np:hasProvenance sub:provenance ;
    np:hasPublicationInfo sub:pubInfo ;
    a np:Nanopublication .
}
sub:assertion {
  obo:CHEBI_16991 rdfs:label "DNA" .
  sub:_1 rdfs:label "pellet" .
  sub:_2 rdfs:label "TE buffer" .
  sub:_3 rdfs:label "nuclease free water" .
  sub:step dce:description "Dissolve the dry pellet in nuclease free water or TE buffer (Ph 8.0). Store DNA at 4 º celcius  or -20 º celcius ." ;
    sp:hasExperimentalOutput obo:CHEBI_16991 ;
    a pplan:Step ;
    bs:bioSampleUsed obo:CHEBI_16991 , sub:_1 ;
    bs:reagentUsed sub:_2 , sub:_3 .
}
sub:provenance {
  sub:assertion pav:createdWith <https://nanotate.bitsfetch.com> ;
    prov:generatedAtTime "2021-03-11T10:45:27.539878"^^xsd:dateTime ;
    prov:wasAttributedTo <https://hypothes.is/users/iannotate> .
}
sub:pubInfo {
  sub:assertion prov:wasDerivedFrom authority:VnoyioKAEeuVQbdsSCpvTg , authority:cE_LhIKAEeuivpusTtTqoA , authority:gLB2hoKAEeupg4ODkkzcpw , authority:ku-e2oKAEeuYs_NH00asTg , authority:p6A23IKAEeuN7qu2B67vEQ .
  sub:sig npx:hasAlgorithm "RSA" ;
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB" ;
    npx:hasSignature "cx663lmiG6ju5rluEf0HxF1Mg0XCdbVXgKznpfQidDhe9ltSZrJT79JQQ7ll6wlA3GqyqPL7vqdeeuvlvbRttbTT1JA1A/48GKgH+1mJRf3oFhQiOUWLaanCsVRA3W9CH1PuEkLd959KvAdG87pUnl2jQoo0d7LHOp6yOlD+IBs=" ;
    npx:hasSignatureTarget this: .
  this: npx:introduces sub:step ;
    prov:generatedAtTime "2021-03-11T10:45:27.539878"^^xsd:dateTime ;
    prov:wasAttributedTo <https://hypothes.is/users/iannotate> .
}