@prefix obo: <
http://purl.obolibrary.org/obo/
> .
@prefix dce: <
http://purl.org/dc/elements/1.1/
> .
@prefix pplan: <
http://purl.org/net/p-plan#
> .
@prefix this: <
http://purl.org/np/RAGVyz0_FLr0Oz6EfGaRgsRbnDJMRqvjWGI72AtFO-jhk
> .
@prefix sub: <
http://purl.org/np/RAGVyz0_FLr0Oz6EfGaRgsRbnDJMRqvjWGI72AtFO-jhk#
> .
@prefix bs: <
https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#
> .
@prefix np: <
http://www.nanopub.org/nschema#
> .
@prefix pav: <
http://purl.org/pav/
> .
@prefix authority: <
https://hypothes.is/a/
> .
@prefix npx: <
http://purl.org/nanopub/x/
> .
@prefix xsd: <
http://www.w3.org/2001/XMLSchema#
> .
@prefix rdfs: <
http://www.w3.org/2000/01/rdf-schema#
> .
@prefix prov: <
http://www.w3.org/ns/prov#
> .
@prefix sp: <
http://purl.org/net/SMARTprotocol#
> .
sub:Head
{
this:
np:hasAssertion
sub:assertion
;
np:hasProvenance
sub:provenance
;
np:hasPublicationInfo
sub:pubInfo
;
a
np:Nanopublication
.
}
sub:assertion
{
obo:CHEBI_16991
rdfs:label
"DNA" .
sub:_1
rdfs:label
"pellet" .
sub:_2
rdfs:label
"TE buffer" .
sub:_3
rdfs:label
"nuclease free water" .
sub:step
dce:description
"Dissolve the dry pellet in nuclease free water or TE buffer (Ph 8.0). Store DNA at 4 º celcius or -20 º celcius ." ;
sp:hasExperimentalOutput
obo:CHEBI_16991
;
a
pplan:Step
;
bs:bioSampleUsed
obo:CHEBI_16991
,
sub:_1
;
bs:reagentUsed
sub:_2
,
sub:_3
.
}
sub:provenance
{
sub:assertion
pav:createdWith
<
https://nanotate.bitsfetch.com
> ;
prov:generatedAtTime
"2021-03-11T10:45:27.539878"^^
xsd:dateTime
;
prov:wasAttributedTo
<
https://hypothes.is/users/iannotate
> .
}
sub:pubInfo
{
sub:assertion
prov:wasDerivedFrom
authority:VnoyioKAEeuVQbdsSCpvTg
,
authority:cE_LhIKAEeuivpusTtTqoA
,
authority:gLB2hoKAEeupg4ODkkzcpw
,
authority:ku-e2oKAEeuYs_NH00asTg
,
authority:p6A23IKAEeuN7qu2B67vEQ
.
sub:sig
npx:hasAlgorithm
"RSA" ;
npx:hasPublicKey
"MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB" ;
npx:hasSignature
"cx663lmiG6ju5rluEf0HxF1Mg0XCdbVXgKznpfQidDhe9ltSZrJT79JQQ7ll6wlA3GqyqPL7vqdeeuvlvbRttbTT1JA1A/48GKgH+1mJRf3oFhQiOUWLaanCsVRA3W9CH1PuEkLd959KvAdG87pUnl2jQoo0d7LHOp6yOlD+IBs=" ;
npx:hasSignatureTarget
this:
.
this:
npx:introduces
sub:step
;
prov:generatedAtTime
"2021-03-11T10:45:27.539878"^^
xsd:dateTime
;
prov:wasAttributedTo
<
https://hypothes.is/users/iannotate
> .
}