@prefix dce: <http://purl.org/dc/elements/1.1/> .
@prefix pplan: <http://purl.org/net/p-plan#> .
@prefix this: <http://purl.org/np/RAHun3lVlaPffCuW0YKIGxrB8WtjZOLSI0NuUCNNgmhtM> .
@prefix sub: <http://purl.org/np/RAHun3lVlaPffCuW0YKIGxrB8WtjZOLSI0NuUCNNgmhtM#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix sp: <http://purl.org/net/SMARTprotocol#> .
sub:Head {
   this: np:hasAssertion sub:assertion ;
    np:hasProvenance sub:provenance ;
    np:hasPublicationInfo sub:pubInfo ;
    a np:Nanopublication .
}
sub:assertion {
   sp:RNAPellet rdfs:label "RNA pellet" .
  sub:_1 rdfs:label "70% RNase-free ethanol" .
  sub:step dce:description "RNA pellet was washed with 1 ml 70% RNase-free ethanol once and spin down by 7,500 x g for 5 min." ;
    sp:hasExperimentalOutput sp:RNAPellet ;
    a pplan:Step ;
    bs:bioSampleUsed sp:RNAPellet ;
    bs:reagentUsed sub:_1 .
}
sub:provenance {
   sub:assertion pav:createdWith <https://nanotate.bitsfetch.com> ;
    prov:generatedAtTime "2021-03-19T15:22:02.652326"^^xsd:dateTime ;
    prov:wasAttributedTo <https://hypothes.is/users/zuluaga.lizzz> .
}
sub:pubInfo {
   sub:assertion prov:wasDerivedFrom authority:3891hGBKEeuKn89HXf7iAg , authority:p5snsmBKEeugLQdOH9C8Yw , authority:w-rUTmBKEeuKnutos7Sd2Q .
  sub:sig npx:hasAlgorithm "RSA" ;
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB" ;
    npx:hasSignature "g18MOVsluohZHED6AxVD4k1nbvuGzQcnsTjQxuc0PdlWMFCAOjPdOav3CWeUmRhkPK3Y+PzZB0sWU+mpLx5Isiv10U70LXtYEIRHLGKDK+imVd8HncAhjyXZ4iYKQ95M2uRACd2Ep7BdBS6yqpNIJYw2D/TYwJNKIw4NjzrD0SM=" ;
    npx:hasSignatureTarget this: .
  this: npx:introduces sub:step ;
    prov:generatedAtTime "2021-03-19T15:22:02.652326"^^xsd:dateTime ;
    prov:wasAttributedTo <https://hypothes.is/users/zuluaga.lizzz> .
}