@prefix obo: <http://purl.obolibrary.org/obo/> .
@prefix dce: <http://purl.org/dc/elements/1.1/> .
@prefix pplan: <http://purl.org/net/p-plan#> .
@prefix this: <http://purl.org/np/RAKpSHKnh8UHKZczlcfbZdI4kJgcAx7985zW8gRt3MOtM> .
@prefix sub: <http://purl.org/np/RAKpSHKnh8UHKZczlcfbZdI4kJgcAx7985zW8gRt3MOtM#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix sp: <http://purl.org/net/SMARTprotocol#> .
sub:Head {
   this: np:hasAssertion sub:assertion ;
    np:hasProvenance sub:provenance ;
    np:hasPublicationInfo sub:pubInfo ;
    a np:Nanopublication .
}
sub:assertion {
   obo:NCBITaxon_1 rdfs:label " root" .
  obo:PO_0025034 rdfs:label "leaf" .
  sub:_1 rdfs:label "recalcitrant samples" .
  sub:_2 rdfs:label "recalcitrant samples" .
  sub:_3 rdfs:label "mortar" .
  sub:_4 rdfs:label "pestle" .
  sub:_5 rdfs:label "extraction buffer" .
  sub:_6 rdfs:label "liquid nitrogen" .
  sub:step dce:description "Ground samples (leaf, shoot, root, and recalcitrant samples, approximately 0.5-1 g) using 1 ml of the extraction buffer with or without liquid nitrogen in mortar and pestle that are sterilized.CRITICAL STEP The procedure are carried out at room temperature except the centrifugation steps (at 4℃) as well as the time of precipitating of the nucleic acid using the isopropanol (on ice).CRITICAL STEP Severely disrupt the tissues to create the glaze mode of samples" ;
    sp:hasExperimentalInput obo:NCBITaxon_1 , obo:PO_0025034 , sub:_1 , <http://www.ebi.ac.uk/efo/EFO_0000992> ;
    a pplan:Step ;
    bs:bioSampleUsed obo:NCBITaxon_1 , obo:PO_0025034 , sub:_2 , <http://www.ebi.ac.uk/efo/EFO_0000992> ;
    bs:labEquipmentUsed sub:_3 , sub:_4 ;
    bs:reagentUsed sub:_5 , sub:_6 .
  <http://www.ebi.ac.uk/efo/EFO_0000992> rdfs:label "shoot" .
}
sub:provenance {
   sub:assertion pav:createdWith <https://nanotate.bitsfetch.com> ;
    prov:generatedAtTime "2021-03-10T10:13:55.313885"^^xsd:dateTime ;
    prov:wasAttributedTo <https://hypothes.is/users/alejandra.sandoval> .
}
sub:pubInfo {
   sub:assertion prov:wasDerivedFrom authority:85nfmHJFEeupsZf00tQnZA , authority:GR0bVHJGEeuiqkcaESmO6Q , authority:OWfHdHJGEeujmAvPIUDH6w , authority:USf8vHJGEeub4tMCkIYnUA , authority:c0g59nJGEeuhuXe3r_IOtA , authority:h_gQsHJGEeujy2Ps728ADg , authority:owhuNnJGEeuFPWP_py-RsA , authority:rbSDonJFEeuo0j902ftpgg , authority:teA4hnJGEeuqgNcqv6DR-w .
  sub:sig npx:hasAlgorithm "RSA" ;
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB" ;
    npx:hasSignature "YMZ4XWZSNv2IF7Nwx7TIa2W+G+wqh1zbsvy8wFq1arAhcaU09B05T5vfvy2LO1xRZh0XwYBy28VwIwl2hJq3fOboDgtotvODg2GWDcnVMxfjqJc/1uib/E8o+TyC75xYRQNkyMSrhqgZzS977Qk9ZPzkv+8Pac0YHLfyJQYU59M=" ;
    npx:hasSignatureTarget this: .
  this: npx:introduces sub:step ;
    prov:generatedAtTime "2021-03-10T10:13:55.313885"^^xsd:dateTime ;
    prov:wasAttributedTo <https://hypothes.is/users/alejandra.sandoval> .
}