sub:assertion {
  obo:OBI_0400155 rdfs:label "water bath" .
  sub:_1 rdfs:label "suspension" .
  sub:_2 rdfs:label "100 mg/ml lysozyme" .
  sub:step dce:description """Bring down temperature of suspension to ~30 °C and add 20 µl of 100 mg/ml lysozyme solution (final concentration, 2 mg/ml) to it. Incubate suspension at 37 °C for 6 h to overnight in a water bath.
Note: In case of fast-growing mycobacteria such as Mycobacterium smegmatis (M. smegmatis), go directly to step 7. However, if DNA is to be isolated from slow-growing species, follow steps 4-6 which involve lysis of cells by a physical method. Because of enhanced disruption of cells, this would significantly enhance yield.""" ;
    a pplan:Step ;
    bs:bioSampleUsed sub:_1 ;
    bs:labEquipmentUsed obo:OBI_0400155 ;
    bs:reagentUsed sub:_2 .
}