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All rights reserved. http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/title BEL Framework Large Corpus Document http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/pav/authoredBy http://www.tkuhn.ch/bel2nanopub/RAMhw2Gmpfez5Gav2gqibGoDW8Cmx-blRcGvRBT_A30qU#_5 http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://purl.org/pav/version 20131211 http://www.tkuhn.ch/bel2nanopub/RAMhw2Gmpfez5Gav2gqibGoDW8Cmx-blRcGvRBT_A30qU#_4 http://www.w3.org/ns/prov#value Plasmids containing ADH3-cat were transfected into HepG2 cells either alone or with expression plasmids for C/EBP-alpha, LAP, DBP, or HNF-1. ADH3 promoter responded quite well to DBP, a very significant synergistic effect for the ADH3 promoter was observed when cells were co-transfected with C/EBP-alpha and LAP expression vectors. The induction levels for ADH3 were 1.5-fold for C/EBP-alpha alone and 3.5-fold for LAP alone but was 20-fold for the mixture (10 microgram of each expression plasmid), indicating a clear synergistic effect. The more significant synergistic effect on ADH3 suggests that C/EBP-alpha and LAP may not be able to effectively transactivate this promoter individually but instead must work together. http://www.tkuhn.ch/bel2nanopub/RAMhw2Gmpfez5Gav2gqibGoDW8Cmx-blRcGvRBT_A30qU#_4 http://www.w3.org/ns/prov#wasQuotedFrom http://www.ncbi.nlm.nih.gov/pubmed/1620113 http://www.tkuhn.ch/bel2nanopub/RAMhw2Gmpfez5Gav2gqibGoDW8Cmx-blRcGvRBT_A30qU#_5 http://www.w3.org/2000/01/rdf-schema#label Selventa http://www.tkuhn.ch/bel2nanopub/RAMhw2Gmpfez5Gav2gqibGoDW8Cmx-blRcGvRBT_A30qU#assertion http://www.w3.org/ns/prov#hadPrimarySource http://www.ncbi.nlm.nih.gov/pubmed/1620113 http://www.tkuhn.ch/bel2nanopub/RAMhw2Gmpfez5Gav2gqibGoDW8Cmx-blRcGvRBT_A30qU#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://resource.belframework.org/belframework/20131211/knowledge/large_corpus.bel http://www.tkuhn.ch/bel2nanopub/RAMhw2Gmpfez5Gav2gqibGoDW8Cmx-blRcGvRBT_A30qU#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://www.tkuhn.ch/bel2nanopub/RAMhw2Gmpfez5Gav2gqibGoDW8Cmx-blRcGvRBT_A30qU#_4 http://www.tkuhn.ch/bel2nanopub/RAMhw2Gmpfez5Gav2gqibGoDW8Cmx-blRcGvRBT_A30qU#pubinfo http://www.tkuhn.ch/bel2nanopub/RAMhw2Gmpfez5Gav2gqibGoDW8Cmx-blRcGvRBT_A30qU http://purl.org/dc/terms/created 2014-07-03T14:32:50.923+02:00 http://www.tkuhn.ch/bel2nanopub/RAMhw2Gmpfez5Gav2gqibGoDW8Cmx-blRcGvRBT_A30qU http://purl.org/pav/createdBy http://orcid.org/0000-0001-6818-334X http://www.tkuhn.ch/bel2nanopub/RAMhw2Gmpfez5Gav2gqibGoDW8Cmx-blRcGvRBT_A30qU http://purl.org/pav/createdBy http://orcid.org/0000-0002-1267-0234