@prefix dce: <http://purl.org/dc/elements/1.1/> .
@prefix pplan: <http://purl.org/net/p-plan#> .
@prefix this: <http://purl.org/np/RAOOw1UKi7ipgdxn7yBsfWDxPy5YS2Iwp_4WpQFDaGWUw> .
@prefix sub: <http://purl.org/np/RAOOw1UKi7ipgdxn7yBsfWDxPy5YS2Iwp_4WpQFDaGWUw#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
sub:Head {
   this: np:hasAssertion sub:assertion ;
    np:hasProvenance sub:provenance ;
    np:hasPublicationInfo sub:pubInfo ;
    a np:Nanopublication .
}
sub:assertion {
   sub:_1 rdfs:label "phenol:chloroform" .
  sub:step dce:description "Add equal volume of phenol:chloroform(1:1) ,shake the tube well for 5 minutes and  centrifuge at 12,000g for 10 minutes at 4º celcius  ." ;
    a pplan:Step ;
    bs:labEquipmentUsed <http://www.bioassayontology.org/bao#BAO_0010014> ;
    bs:reagentUsed sub:_1 .
  <http://www.bioassayontology.org/bao#BAO_0010014> rdfs:label "tube" .
}
sub:provenance {
   sub:assertion pav:createdWith <https://nanotate.bitsfetch.com> ;
    prov:generatedAtTime "2021-03-11T10:50:24.056338"^^xsd:dateTime ;
    prov:wasAttributedTo <https://hypothes.is/users/iannotate> .
}
sub:pubInfo {
   sub:assertion prov:wasDerivedFrom authority:KoICRIJ_EeuSbp-myt8ckg , authority:QXPNXIJ_Eeu4-W-HbiVXFQ , authority:U3_9GIJ_EeuMEHc4qpHi8Q .
  sub:sig npx:hasAlgorithm "RSA" ;
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB" ;
    npx:hasSignature "ZdbOskvtXAhji6WTmW2bSHio13TT6u0vX6jG0byWezQjALj3bp4uPaS56TAhnA/HcwbmOCMOEwxD4Zx/1XWwuUDZXm0hKJcfxoVKF53W45IPUh6h2jbPAvJnABO70P1OqQfGeswJDkzP9U5oydE7ddbPphGpmEJe5DwzJkCW4vY=" ;
    npx:hasSignatureTarget this: .
  this: npx:introduces sub:step ;
    prov:generatedAtTime "2021-03-11T10:50:24.056338"^^xsd:dateTime ;
    prov:wasAttributedTo <https://hypothes.is/users/iannotate> .
}