@prefix dce: <http://purl.org/dc/elements/1.1/> .
@prefix pplan: <http://purl.org/net/p-plan#> .
@prefix this: <http://purl.org/np/RAPAePEKsqNCQUMbicaLxC1HPXUduUzsonZfiz1Bsr6Oo> .
@prefix sub: <http://purl.org/np/RAPAePEKsqNCQUMbicaLxC1HPXUduUzsonZfiz1Bsr6Oo#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix sp: <http://purl.org/net/SMARTprotocol#> .
sub:Head {
   this: np:hasAssertion sub:assertion ;
    np:hasProvenance sub:provenance ;
    np:hasPublicationInfo sub:pubInfo ;
    a np:Nanopublication .
}
sub:assertion {
   sub:_1 rdfs:label "preserved mosquitoes" .
  sub:_2 rdfs:label "preserved mosquitoes" .
  sub:_3 rdfs:label "phosphate buffer saline(PBS)" .
  sub:_4 rdfs:label "sterile distilled water " .
  sub:step dce:description "Wash preserved mosquitoes in sterile distilled water or phosphate buffer saline(PBS) to remove excess alcohol. Fresh mosquitoes can be ground directly." ;
    sp:hasExperimentalInput sub:_1 ;
    a pplan:Step ;
    bs:bioSampleUsed sub:_2 ;
    bs:reagentUsed sub:_3 , sub:_4 .
}
sub:provenance {
   sub:assertion pav:createdWith <https://nanotate.bitsfetch.com> ;
    prov:generatedAtTime "2021-03-10T10:34:49.508648"^^xsd:dateTime ;
    prov:wasAttributedTo <https://hypothes.is/users/alejandra.sandoval> .
}
sub:pubInfo {
   sub:assertion prov:wasDerivedFrom authority:1F5Y7HJLEeupBj83tIGoYA , authority:7-EONHJLEeugRO_LycGZxA , authority:BpknYHJMEeu9FrvRcZqR2g , authority:qelCtnJLEeumETt60OJmtQ .
  sub:sig npx:hasAlgorithm "RSA" ;
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB" ;
    npx:hasSignature "O3+r7mQpO3NWmypA7SyC58HfZOGPxDZrAeDxo5C6pObBr3gAtY+5mrEBBUySAqG+uB3eHi3Wax/c5gYcoXNgoDD38I4sxSNEeVYHFQa5U0fSWU7zajKJFajAMhBPru34+xF4H0lINhCL6bsdVYFtrZUUdQY3H3Q03QIoKcqxM9Q=" ;
    npx:hasSignatureTarget this: .
  this: npx:introduces sub:step ;
    prov:generatedAtTime "2021-03-10T10:34:49.508648"^^xsd:dateTime ;
    prov:wasAttributedTo <https://hypothes.is/users/alejandra.sandoval> .
}