@prefix obo: <http://purl.obolibrary.org/obo/> .
@prefix dce: <http://purl.org/dc/elements/1.1/> .
@prefix pplan: <http://purl.org/net/p-plan#> .
@prefix this: <http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY> .
@prefix sub: <http://purl.org/np/RAPJMxwqkQuu25zfvPcDry7DWNX8yqWWmFdBPNX-LP2nY#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
sub:Head {
   this: np:hasAssertion sub:assertion ;
    np:hasProvenance sub:provenance ;
    np:hasPublicationInfo sub:pubInfo ;
    a np:Nanopublication .
}
sub:assertion {
   obo:CHEBI_26710 rdfs:label "5 M NaCl" .
  sub:_1 rdfs:label "supernatant" .
  sub:_2 rdfs:label "chloroform: isoamyl alcohol" .
  sub:_3 rdfs:label "RNAse A" .
  sub:step dce:description "After RNAse A treatment, add one volume of chloroform: isoamyl alcohol again and mix by inversions for 5 min. The tube is centrifuged for 10 min at 5000 × g at 4°C. Transfer the clear supernatant into new 2ml tube and add half volume of 5 M NaCl to the sample and mix gently by inversions." ;
    a pplan:Step ;
    bs:bioSampleUsed sub:_1 ;
    bs:labEquipmentUsed <http://www.bioassayontology.org/bao#BAO_0010014> ;
    bs:reagentUsed obo:CHEBI_26710 , sub:_2 , sub:_3 .
  <http://www.bioassayontology.org/bao#BAO_0010014> rdfs:label "tube" .
}
sub:provenance {
   sub:assertion pav:createdWith <https://nanotate.bitsfetch.com> ;
    prov:generatedAtTime "2021-03-11T16:42:19.939476"^^xsd:dateTime ;
    prov:wasAttributedTo <https://hypothes.is/users/iannotate> .
}
sub:pubInfo {
   sub:assertion prov:wasDerivedFrom authority:65gnrIKwEeu8ozv-JryAeQ , authority:E7j6IoKxEeuOsU_XwyDh1w , authority:LAccdoKxEeuiRiOOl5XFJA , authority:SUBJ6IKxEeuOTeteBAH1mw , authority:_rt9SIKwEeuOS7PRsXqlQA , authority:nLN1sIKwEeufn8eMqZYi6g .
  sub:sig npx:hasAlgorithm "RSA" ;
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB" ;
    npx:hasSignature "cCe5S4PTfHVNKbJDNVTTQAqxqGXwmCdNl2CL+z9K1r5knTrma1JscEMcqbOvXXiK6IXqUJ8SJ22C1RFBjxCLccSsoInb79UamARsI2c6Zh248oaxNEsSHF8BKLmWGwwDTKEZ1oqpZ7j4igSflkPVAUQ06IgSP1s6STvxRc3jNJs=" ;
    npx:hasSignatureTarget this: .
  this: npx:introduces sub:step ;
    prov:generatedAtTime "2021-03-11T16:42:19.939476"^^xsd:dateTime ;
    prov:wasAttributedTo <https://hypothes.is/users/iannotate> .
}