@prefix dce: <
http://purl.org/dc/elements/1.1/
> .
@prefix pplan: <
http://purl.org/net/p-plan#
> .
@prefix this: <
http://purl.org/np/RAPh7iwyYLWtP9Efe7f9vmAT1nI-dcwaH_miAMP66yKP8
> .
@prefix sub: <
http://purl.org/np/RAPh7iwyYLWtP9Efe7f9vmAT1nI-dcwaH_miAMP66yKP8#
> .
@prefix bs: <
https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#
> .
@prefix np: <
http://www.nanopub.org/nschema#
> .
@prefix pav: <
http://purl.org/pav/
> .
@prefix authority: <
https://hypothes.is/a/
> .
@prefix npx: <
http://purl.org/nanopub/x/
> .
@prefix xsd: <
http://www.w3.org/2001/XMLSchema#
> .
@prefix rdfs: <
http://www.w3.org/2000/01/rdf-schema#
> .
@prefix prov: <
http://www.w3.org/ns/prov#
> .
sub:Head
{
this:
np:hasAssertion
sub:assertion
;
np:hasProvenance
sub:provenance
;
np:hasPublicationInfo
sub:pubInfo
;
a
np:Nanopublication
.
}
sub:assertion
{
<
http://purl.bioontology.org/ontology/NCBITAXON/2
>
rdfs:label
"bacteria" .
sub:_1
rdfs:label
"pestle" .
sub:_2
rdfs:label
"mortar" .
sub:_3
rdfs:label
"liquid N2" .
sub:step
dce:description
"Snap freeze pellet in liquid N2 and immediately transfer to a mortar. Using a pestle, crush bacteria while repeatedly adding liquid N2 (~20 ml) to it." ;
a
pplan:Step
;
bs:bioSampleUsed
<
http://purl.bioontology.org/ontology/NCBITAXON/2
> ;
bs:labEquipmentUsed
sub:_1
,
sub:_2
;
bs:reagentUsed
sub:_3
.
}
sub:provenance
{
sub:assertion
pav:createdWith
<
https://nanotate.bitsfetch.com
> ;
prov:generatedAtTime
"2021-02-26T09:42:50.267128"^^
xsd:dateTime
;
prov:wasAttributedTo
<
https://hypothes.is/users/zuluaga.lizzz
> .
}
sub:pubInfo
{
sub:assertion
prov:wasDerivedFrom
authority:HF6vXnezEeu9Nm9sG2yvxA
,
authority:K_6wnnezEeu6xFvlmS3RLw
,
authority:PMMhOnezEeuugL9mos-sdg
,
authority:SwUHhnezEeullruxPC8PDw
,
authority:XqW5PnezEeuhbOsMqr97sQ
.
sub:sig
npx:hasAlgorithm
"RSA" ;
npx:hasPublicKey
"MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB" ;
npx:hasSignature
"IfnCkjk1yQ3uFlDOk0/RdKMY8E/nD5TlmtusVBjmSYTXoWmpnk7MtKnOXpKAeq9SqVUsBx3gNaUceKALRxII5pYYTISHS5dJ6nOu+MT/YgRMcotbfqUsH7f+xFeFGKRRaGATsEGQvCKXG1NLZoU/pxcBGtsIw5JAaVlKO8ybZzc=" ;
npx:hasSignatureTarget
this:
.
this:
npx:introduces
sub:step
;
prov:generatedAtTime
"2021-02-26T09:42:50.267128"^^
xsd:dateTime
;
prov:wasAttributedTo
<
https://hypothes.is/users/zuluaga.lizzz
> .
}