@prefix dce: <http://purl.org/dc/elements/1.1/> .
@prefix pplan: <http://purl.org/net/p-plan#> .
@prefix this: <http://purl.org/np/RAPh7iwyYLWtP9Efe7f9vmAT1nI-dcwaH_miAMP66yKP8> .
@prefix sub: <http://purl.org/np/RAPh7iwyYLWtP9Efe7f9vmAT1nI-dcwaH_miAMP66yKP8#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
sub:Head {
  this: np:hasAssertion sub:assertion ;
    np:hasProvenance sub:provenance ;
    np:hasPublicationInfo sub:pubInfo ;
    a np:Nanopublication .
}
sub:assertion {
  <http://purl.bioontology.org/ontology/NCBITAXON/2> rdfs:label "bacteria" .
  sub:_1 rdfs:label "pestle" .
  sub:_2 rdfs:label "mortar" .
  sub:_3 rdfs:label "liquid N2" .
  sub:step dce:description "Snap freeze pellet in liquid N2 and immediately transfer to a mortar. Using a pestle, crush bacteria while repeatedly adding liquid N2 (~20 ml) to it." ;
    a pplan:Step ;
    bs:bioSampleUsed <http://purl.bioontology.org/ontology/NCBITAXON/2> ;
    bs:labEquipmentUsed sub:_1 , sub:_2 ;
    bs:reagentUsed sub:_3 .
}
sub:provenance {
  sub:assertion pav:createdWith <https://nanotate.bitsfetch.com> ;
    prov:generatedAtTime "2021-02-26T09:42:50.267128"^^xsd:dateTime ;
    prov:wasAttributedTo <https://hypothes.is/users/zuluaga.lizzz> .
}
sub:pubInfo {
  sub:assertion prov:wasDerivedFrom authority:HF6vXnezEeu9Nm9sG2yvxA , authority:K_6wnnezEeu6xFvlmS3RLw , authority:PMMhOnezEeuugL9mos-sdg , authority:SwUHhnezEeullruxPC8PDw , authority:XqW5PnezEeuhbOsMqr97sQ .
  sub:sig npx:hasAlgorithm "RSA" ;
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB" ;
    npx:hasSignature "IfnCkjk1yQ3uFlDOk0/RdKMY8E/nD5TlmtusVBjmSYTXoWmpnk7MtKnOXpKAeq9SqVUsBx3gNaUceKALRxII5pYYTISHS5dJ6nOu+MT/YgRMcotbfqUsH7f+xFeFGKRRaGATsEGQvCKXG1NLZoU/pxcBGtsIw5JAaVlKO8ybZzc=" ;
    npx:hasSignatureTarget this: .
  this: npx:introduces sub:step ;
    prov:generatedAtTime "2021-02-26T09:42:50.267128"^^xsd:dateTime ;
    prov:wasAttributedTo <https://hypothes.is/users/zuluaga.lizzz> .
}