@prefix pplan: <http://purl.org/net/p-plan#> .
@prefix dce: <http://purl.org/dc/elements/1.1/> .
@prefix this: <http://purl.org/np/RATUKFz980ttcIBG3zkIhrgSqY5vqL4Ea7Fr1B20PY9rI> .
@prefix sub: <http://purl.org/np/RATUKFz980ttcIBG3zkIhrgSqY5vqL4Ea7Fr1B20PY9rI#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix sp: <http://purl.org/net/SMARTprotocol#> .
sub:Head {
   this: np:hasAssertion sub:assertion ;
    np:hasProvenance sub:provenance ;
    np:hasPublicationInfo sub:pubInfo ;
    a np:Nanopublication .
}
sub:assertion {
   sub:_1 rdfs:label "Cells" .
  sub:_2 rdfs:label "Cells" .
  sub:step dce:description """Cells can be treated with different antigens for different period of times and the supernatants can be analysed for cytokine levels. The cells can be analysed for phenotypic change, apoptosis or proliferation.
Note: PBMCs are primary cells and cannot be cultured for more than one passage under normal conditions. Lymphocytes of PBMCs can be made to proliferate in vitro by mitogens e.g., Phytohaemagglutin or Concanavin-A etc over a period to 72-96 h. Monocytes generally are end cells and do not proliferate. In absence of mitogens the proliferation of PBMCs will be negligible.""" ;
    sp:hasExperimentalOutput sub:_1 ;
    a pplan:Step ;
    bs:bioSampleUsed sub:_2 .
}
sub:provenance {
   sub:assertion pav:createdWith <https://nanotate.bitsfetch.com> ;
    prov:generatedAtTime "2021-03-05T10:02:33.868997"^^xsd:dateTime ;
    prov:wasAttributedTo <https://hypothes.is/users/iannotate> .
}
sub:pubInfo {
   sub:assertion prov:wasDerivedFrom authority:H1Jtbn0qEeuSC7PM2bR4uQ , authority:LPyjxn0qEeu4p_vSx7_C9Q .
  sub:sig npx:hasAlgorithm "RSA" ;
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB" ;
    npx:hasSignature "ZdRZzTMSqU206YBeBHIPTZpSmUTgiqzqwdkDl1G9rR1UzxQ2lsd4C+a9o1zesZ8yPjsHjpDLZj1nGD0w7I7K+pDZG5fbulT16WxVP46qJAYsR47vryc3CZg9vtArie8TjphFXN2ZUGA+FNTHEVoNUqEGbYWbRE3pwK1duoJ1DLE=" ;
    npx:hasSignatureTarget this: .
  this: npx:introduces sub:step ;
    prov:generatedAtTime "2021-03-05T10:02:33.868997"^^xsd:dateTime ;
    prov:wasAttributedTo <https://hypothes.is/users/iannotate> .
}