@prefix pplan: <
http://purl.org/net/p-plan#
> .
@prefix dce: <
http://purl.org/dc/elements/1.1/
> .
@prefix this: <
http://purl.org/np/RAUUccS7ZcbbSzOS-zqgDJ6IDdTbax_9TPhR4FMyoUOtA
> .
@prefix sub: <
http://purl.org/np/RAUUccS7ZcbbSzOS-zqgDJ6IDdTbax_9TPhR4FMyoUOtA#
> .
@prefix bs: <
https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#
> .
@prefix np: <
http://www.nanopub.org/nschema#
> .
@prefix pav: <
http://purl.org/pav/
> .
@prefix authority: <
https://hypothes.is/a/
> .
@prefix npx: <
http://purl.org/nanopub/x/
> .
@prefix xsd: <
http://www.w3.org/2001/XMLSchema#
> .
@prefix rdfs: <
http://www.w3.org/2000/01/rdf-schema#
> .
@prefix prov: <
http://www.w3.org/ns/prov#
> .
@prefix sp: <
http://purl.org/net/SMARTprotocol#
> .
sub:Head
{
this:
np:hasAssertion
sub:assertion
;
np:hasProvenance
sub:provenance
;
np:hasPublicationInfo
sub:pubInfo
;
a
np:Nanopublication
.
}
sub:assertion
{
<
http://purl.obolibrary.org/obo/CHEBI_16991
>
rdfs:label
"DNA" .
sub:_1
rdfs:label
"pellet" .
sub:_2
rdfs:label
"pellet" .
sub:_3
rdfs:label
"nuclease free water" .
sub:_4
rdfs:label
"TE buffer" .
sub:step
dce:description
"Dissolve the dry pellet in nuclease free water or TE buffer (Ph 8.0). Store DNA at 4 º celcius or -20 º celcius ." ;
sp:hasExperimentalOutput
<
http://purl.obolibrary.org/obo/CHEBI_16991
> ,
sub:_1
;
a
pplan:Step
;
bs:bioSampleUsed
<
http://purl.obolibrary.org/obo/CHEBI_16991
> ,
sub:_2
;
bs:reagentUsed
sub:_3
,
sub:_4
.
}
sub:provenance
{
sub:assertion
pav:createdWith
<
https://nanotate.bitsfetch.com
> ;
prov:generatedAtTime
"2021-02-24T15:55:41.254735"^^
xsd:dateTime
;
prov:wasAttributedTo
<
https://hypothes.is/users/zuluaga.lizzz
> .
}
sub:pubInfo
{
sub:assertion
prov:wasDerivedFrom
authority:IMGwSnbiEeuyhtu9n7srbg
,
authority:NDLvkHbiEeuvGws9CpXYEg
,
authority:RxAaSHbiEeuf9Qs7D-7mbQ
,
authority:W5TnvnbiEeu9zJfZKE5SbA
,
authority:cQA86HbiEeuDoKPmfzScPg
.
sub:sig
npx:hasAlgorithm
"RSA" ;
npx:hasPublicKey
"MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB" ;
npx:hasSignature
"AHJ5Yc8Tzak1K5M6ymjq/Lx8OO2o5lM3p94CzafH5Ni0VOBC/azC+NBC97XRdzkbhQZ4MweCnsIMI6wZLEIdTPaziFpTvDx6EuDyaonhR3iTRXpGg1mHvgoaUCIyt3vudun5cVc0SIyFfCdUlKtB5OtwrVgMjXRJt+ug1mdMzh4=" ;
npx:hasSignatureTarget
this:
.
this:
npx:introduces
sub:step
;
prov:generatedAtTime
"2021-02-24T15:55:41.254735"^^
xsd:dateTime
;
prov:wasAttributedTo
<
https://hypothes.is/users/zuluaga.lizzz
> .
}