@prefix pplan: <
http://purl.org/net/p-plan#
> .
@prefix dce: <
http://purl.org/dc/elements/1.1/
> .
@prefix this: <
http://purl.org/np/RAUfXaNwZwQALTgebIITnK7ysM-tPNuQ75VFKD0SbBlu0
> .
@prefix sub: <
http://purl.org/np/RAUfXaNwZwQALTgebIITnK7ysM-tPNuQ75VFKD0SbBlu0#
> .
@prefix bs: <
https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#
> .
@prefix np: <
http://www.nanopub.org/nschema#
> .
@prefix pav: <
http://purl.org/pav/
> .
@prefix authority: <
https://hypothes.is/a/
> .
@prefix npx: <
http://purl.org/nanopub/x/
> .
@prefix xsd: <
http://www.w3.org/2001/XMLSchema#
> .
@prefix rdfs: <
http://www.w3.org/2000/01/rdf-schema#
> .
@prefix prov: <
http://www.w3.org/ns/prov#
> .
@prefix sp: <
http://purl.org/net/SMARTprotocol#
> .
sub:Head
{
this:
np:hasAssertion
sub:assertion
;
np:hasProvenance
sub:provenance
;
np:hasPublicationInfo
sub:pubInfo
;
a
np:Nanopublication
.
}
sub:assertion
{
<
http://purl.obolibrary.org/obo/CHEBI_16991
>
rdfs:label
"DNA" .
<
http://purl.obolibrary.org/obo/CHEBI_17824
>
rdfs:label
" isopropanol" .
<
http://purl.obolibrary.org/obo/CHEBI_33697
>
rdfs:label
"RNA" .
sub:_1
rdfs:label
"tubes" .
sub:step
dce:description
"Add 700 µl of cold isopropanol to precipitate RNA or DNA and then invert tubes 3-4 times to mix the solution" ;
sp:hasExperimentalOutput
<
http://purl.obolibrary.org/obo/CHEBI_16991
> , <
http://purl.obolibrary.org/obo/CHEBI_33697
> ;
a
pplan:Step
;
bs:bioSampleUsed
<
http://purl.obolibrary.org/obo/CHEBI_16991
> , <
http://purl.obolibrary.org/obo/CHEBI_33697
> ;
bs:labEquipmentUsed
sub:_1
;
bs:reagentUsed
<
http://purl.obolibrary.org/obo/CHEBI_17824
> .
}
sub:provenance
{
sub:assertion
pav:createdWith
<
https://nanotate.bitsfetch.com
> ;
prov:generatedAtTime
"2021-03-10T10:23:28.749259"^^
xsd:dateTime
;
prov:wasAttributedTo
<
https://hypothes.is/users/alejandra.sandoval
> .
}
sub:pubInfo
{
sub:assertion
prov:wasDerivedFrom
authority:P5RZ1HJJEeuWar_pp0JPJg
,
authority:Wj6gnoG0EeuDiLcw3qKtQA
,
authority:amrONnJJEeu0w_eniipiDg
,
authority:iKR-QnJJEeuOYP-b54Owxg
,
authority:z1ykbnJIEeu-7tMG2ROcBQ
.
sub:sig
npx:hasAlgorithm
"RSA" ;
npx:hasPublicKey
"MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB" ;
npx:hasSignature
"eCorSCSXkCljbtieRN4QpD7krTPK2MV3lIuh6e/pnBR+LKfA9ytleKulc/debTZVXpBb9nu4s+2jGHqx1rxJbmFiSyIBqxfwReYj+bHsmhz/CuK59GgwRpQ3T3RoitqH5O2rPtbxgG+s3P4kIXzFAJSRwjdRc6+kZDObrqGj09Y=" ;
npx:hasSignatureTarget
this:
.
this:
npx:introduces
sub:step
;
prov:generatedAtTime
"2021-03-10T10:23:28.749259"^^
xsd:dateTime
;
prov:wasAttributedTo
<
https://hypothes.is/users/alejandra.sandoval
> .
}