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All rights reserved. http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/title BEL Framework Large Corpus Document http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/pav/authoredBy http://www.tkuhn.ch/bel2nanopub/RAd_xMO2VqTdhIrQjlvedli2Vjua6wQpxvBJpdHeepO7M#_5 http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/pav/version 1.4 http://www.tkuhn.ch/bel2nanopub/RAd_xMO2VqTdhIrQjlvedli2Vjua6wQpxvBJpdHeepO7M#_4 http://www.w3.org/ns/prov#value Mammary epithelial 31EG4 cells (MEC) were grown as monolayers on filters to analyze the apical membrane mechanisms that help mediate ion and fluid transport across the epithelium. RT-PCR showed the presence of cystic fibrosis transmembrane conductance regulator (CFTR) and epithelial Na(+) channel (ENaC) message, and immunomicroscopy showed apical membrane staining for both proteins. CFTR was also localized to the apical membrane of native human mammary duct epithelium. In control conditions, mean values of transepithelial potential (apical-side negative) and resistance (R(T)) are -5.9 mV and 829 Omega x cm(2), respectively. The apical membrane potential (V(A)) is -40.7 mV, and the mean ratio of apical to basolateral membrane resistance (R(A)/R(B)) is 2.8. Apical amiloride hyperpolarized V(A) by 19.7 mV and tripled R(A)/R(B). A cAMP-elevating cocktail depolarized V(A) by 17.6 mV, decreased R(A)/R(B) by 60%, increased short-circuit current by 6 microA/cm(2), decreased R(T) by 155 Omega x cm(2), and largely eliminated responses to amiloride. Whole cell patch-clamp measurements demonstrated amiloride-inhibited Na(+) currents [linear current-voltage (I-V) relation] and forskolin-stimulated Cl(-) currents (linear I-V relation). A capacitance probe method showed that in the control state, MEC monolayers either absorbed or secreted fluid (2--4 microl x cm(-2) x h(-1)). Fluid secretion was stimulated either by activating CFTR (cAMP) or blocking ENaC (amiloride). These data plus equivalent circuit analysis showed that 1) fluid absorption across MEC is mediated by Na(+) transport via apical membrane ENaC, and fluid secretion is mediated, in part, by Cl(-) transport via apical CFTR; 2) in both cases, appropriate counterions move through tight junctions to maintain electroneutrality; and 3) interactions among CFTR, ENaC, and tight junctions allow MEC to either absorb or secrete fluid and, in situ, may help control luminal [Na(+)] and [Cl(-)]. http://www.tkuhn.ch/bel2nanopub/RAd_xMO2VqTdhIrQjlvedli2Vjua6wQpxvBJpdHeepO7M#_4 http://www.w3.org/ns/prov#wasQuotedFrom http://www.ncbi.nlm.nih.gov/pubmed/11443063 http://www.tkuhn.ch/bel2nanopub/RAd_xMO2VqTdhIrQjlvedli2Vjua6wQpxvBJpdHeepO7M#_5 http://www.w3.org/2000/01/rdf-schema#label Selventa http://www.tkuhn.ch/bel2nanopub/RAd_xMO2VqTdhIrQjlvedli2Vjua6wQpxvBJpdHeepO7M#assertion http://www.w3.org/ns/prov#hadPrimarySource http://www.ncbi.nlm.nih.gov/pubmed/11443063 http://www.tkuhn.ch/bel2nanopub/RAd_xMO2VqTdhIrQjlvedli2Vjua6wQpxvBJpdHeepO7M#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://www.tkuhn.ch/bel2nanopub/RAd_xMO2VqTdhIrQjlvedli2Vjua6wQpxvBJpdHeepO7M#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://www.tkuhn.ch/bel2nanopub/RAd_xMO2VqTdhIrQjlvedli2Vjua6wQpxvBJpdHeepO7M#_4 http://www.tkuhn.ch/bel2nanopub/RAd_xMO2VqTdhIrQjlvedli2Vjua6wQpxvBJpdHeepO7M#pubinfo http://www.tkuhn.ch/bel2nanopub/RAd_xMO2VqTdhIrQjlvedli2Vjua6wQpxvBJpdHeepO7M http://purl.org/dc/terms/created 2014-07-03T14:29:57.180+02:00 http://www.tkuhn.ch/bel2nanopub/RAd_xMO2VqTdhIrQjlvedli2Vjua6wQpxvBJpdHeepO7M http://purl.org/pav/createdBy http://orcid.org/0000-0001-6818-334X http://www.tkuhn.ch/bel2nanopub/RAd_xMO2VqTdhIrQjlvedli2Vjua6wQpxvBJpdHeepO7M http://purl.org/pav/createdBy http://orcid.org/0000-0002-1267-0234