@prefix pplan: <http://purl.org/net/p-plan#> .
@prefix dce: <http://purl.org/dc/elements/1.1/> .
@prefix this: <http://purl.org/np/RAeY0Vlxxi1oQQ1OwNafr-5UU5CS1YjZpAzAPO1gTSQtQ> .
@prefix sub: <http://purl.org/np/RAeY0Vlxxi1oQQ1OwNafr-5UU5CS1YjZpAzAPO1gTSQtQ#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix sp: <http://purl.org/net/SMARTprotocol#> .
sub:Head {
   this: np:hasAssertion sub:assertion ;
    np:hasProvenance sub:provenance ;
    np:hasPublicationInfo sub:pubInfo ;
    a np:Nanopublication .
}
sub:assertion {
   <http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C62218> rdfs:label "70% ethanol" .
  <http://purl.obolibrary.org/obo/CHEBI_16991> rdfs:label "DNA" .
  sub:_1 rdfs:label "pellet" .
  sub:_2 rdfs:label "tubes" .
  sub:_3 rdfs:label "TE" .
  sub:step dce:description "After incubation, the tubes were centrifuged at 5000 × g for 10 min at 4°C and the supernatant was gently removed. The pellet is washed two times with 1ml of 70% ethanol and the DNA is pellet by 5000 × g at 4°C for only 5 min. The supernatant is discarded and the pellet is air-dried (10 min). The pellet are allowed to re-suspend in 50 μL of TE (10 mM Tris. HCl pH 8.0; 1 mM EDTA pH 8.0)." ;
    sp:hasExperimentalOutput <http://purl.obolibrary.org/obo/CHEBI_16991> ;
    a pplan:Step ;
    bs:bioSampleUsed <http://purl.obolibrary.org/obo/CHEBI_16991> , sub:_1 ;
    bs:labEquipmentUsed sub:_2 ;
    bs:reagentUsed <http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C62218> , sub:_3 .
}
sub:provenance {
   sub:assertion pav:createdWith <https://nanotate.bitsfetch.com> ;
    prov:generatedAtTime "2021-03-11T16:39:49.461208"^^xsd:dateTime ;
    prov:wasAttributedTo <https://hypothes.is/users/iannotate> .
}
sub:pubInfo {
   sub:assertion prov:wasDerivedFrom <https://hypothes.is/a/-I88TIKxEeu85fuKGtcBIw> , authority:4n20poKxEeu9i_eN_Ca7Dw , authority:DkWQSoKyEeuQBW-OJG3rAQ , authority:KTUEYoKyEeuz26fBERP8ow , authority:u6u4PIKxEeuEqgdWCyMzjQ , authority:zsdcWoKxEeun64_lb_lpTg .
  sub:sig npx:hasAlgorithm "RSA" ;
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB" ;
    npx:hasSignature "WSksEo+95kLDcq4ezOI8iCMgFiMU4KLiLWhZquNxfHGJDW5UHUogYpmP0TczC4QUlpJC92X4k0iD2qF6sn6bETrWDeLagvjsZgE8MGESraGLGkK8pAoSnewc5icmi28O2jXUqNMjBCNRa1RmQmQjSVqXQFM/Im6mZWaf8gZIdGM=" ;
    npx:hasSignatureTarget this: .
  this: npx:introduces sub:step ;
    prov:generatedAtTime "2021-03-11T16:39:49.461208"^^xsd:dateTime ;
    prov:wasAttributedTo <https://hypothes.is/users/iannotate> .
}