@prefix pplan: <
http://purl.org/net/p-plan#
> .
@prefix dce: <
http://purl.org/dc/elements/1.1/
> .
@prefix this: <
http://purl.org/np/RAea1HarqPKjTqaYCgFAZIHurIPMDCvVfekwWQBiPEv_g
> .
@prefix sub: <
http://purl.org/np/RAea1HarqPKjTqaYCgFAZIHurIPMDCvVfekwWQBiPEv_g#
> .
@prefix bs: <
https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#
> .
@prefix np: <
http://www.nanopub.org/nschema#
> .
@prefix pav: <
http://purl.org/pav/
> .
@prefix authority: <
https://hypothes.is/a/
> .
@prefix npx: <
http://purl.org/nanopub/x/
> .
@prefix xsd: <
http://www.w3.org/2001/XMLSchema#
> .
@prefix rdfs: <
http://www.w3.org/2000/01/rdf-schema#
> .
@prefix prov: <
http://www.w3.org/ns/prov#
> .
@prefix sp: <
http://purl.org/net/SMARTprotocol#
> .
sub:Head
{
this:
np:hasAssertion
sub:assertion
;
np:hasProvenance
sub:provenance
;
np:hasPublicationInfo
sub:pubInfo
;
a
np:Nanopublication
.
}
sub:assertion
{
<
http://purl.obolibrary.org/obo/CHEBI_16991
>
rdfs:label
"DNA" .
<
http://purl.obolibrary.org/obo/CHEBI_17824
>
rdfs:label
" isopropanol" .
sub:step
dce:description
"Add an equal volume of isopropanol to tube. Mix contents gently and keep undisturbed for 5-10 min. The white thread-like structure of precipitated DNA will be seen in suspension" ;
sp:hasExperimentalOutput
<
http://purl.obolibrary.org/obo/CHEBI_16991
> ;
a
pplan:Step
;
bs:bioSampleUsed
<
http://purl.obolibrary.org/obo/CHEBI_16991
> ;
bs:labEquipmentUsed
<
http://www.bioassayontology.org/bao#BAO_0010014
> ;
bs:reagentUsed
<
http://purl.obolibrary.org/obo/CHEBI_17824
> .
<
http://www.bioassayontology.org/bao#BAO_0010014
>
rdfs:label
"tube" .
}
sub:provenance
{
sub:assertion
pav:createdWith
<
https://nanotate.bitsfetch.com
> ;
prov:generatedAtTime
"2021-02-26T09:46:53.028236"^^
xsd:dateTime
;
prov:wasAttributedTo
<
https://hypothes.is/users/zuluaga.lizzz
> .
}
sub:pubInfo
{
sub:assertion
prov:wasDerivedFrom
authority:5c6XWng_EeufV4Mf5WmEkA
,
authority:PR5n5Hg-EeusjNPPSRf4PQ
,
authority:U-5CRng-EeuRLaefFZBjvg
,
authority:d0B2dHg-EeufUIP78yBROQ
.
sub:sig
npx:hasAlgorithm
"RSA" ;
npx:hasPublicKey
"MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB" ;
npx:hasSignature
"b47qol9W+RwuLX1SAOsGtadNeonj0pZTNtNV+k5dB71Oyu9CKFOE98AqBL3ZOct4pKGO2+eHVMwhY6CvC2lHiZ9HwKInsW+NqDC9NdCI9B9fq3rLobmqNeZi3//sIQrnGTO3oopqzS3n/0CFcsdLqq1o3puvE8wBLiBZ85855/Y=" ;
npx:hasSignatureTarget
this:
.
this:
npx:introduces
sub:step
;
prov:generatedAtTime
"2021-02-26T09:46:53.028236"^^
xsd:dateTime
;
prov:wasAttributedTo
<
https://hypothes.is/users/zuluaga.lizzz
> .
}